The adaptation of a tetrazolium salt assay for serum lactate dehydrogenase to full automation is described. The tetrazolium salt, INT, (2-p-iodophenyl-3-p-nitrophenyl5-phenyl tetrazolium chloride) serves as the chromogenic indicator of the enzyme activity. Phenazine methosulfate serves as an intermediate electron carrier. Estimation requires only 5 mm. of incubation, and assays may be run at th...

Bacteria belonging to the Alphaproteobacteria normally harbour multiple copies of the heat shock sigma factor (known as σ(32), σ(H) or RpoH). Azospirillum brasilense, a non-photosynthetic rhizobacterium, harbours five copies of rpoH genes, one of which is an rpoH2 homologue. The genes around the rpoH2 locus in A. brasilense show synteny with that found in rhizobia. The rpoH2 of A. brasilense wa...

We coupled 16S rDNA PCR and DNA hybridization technology to construct a microarray for simultaneous detection and discrimination of eight fish pathogens (Aeromonas hydrophila, Edwardsiella tarda, Flavobacterium columnare, Lactococcus garvieae, Photobacterium damselae, Pseudomonas anguilliseptica, Streptococcus iniae and Vibrio anguillarum) commonly encountered in aquaculture. The array comprise...

Cancer is a disease in which there is an uncontrolled multiplication and spread, within the body, of abnormal forms of the body’s own cells. Assessment of In-vitro cytotoxicity has been recently become popular as a primary screening method for evaluating anticancer activities of various natural source. This study was carried out to characterize anti-cancer activity of methanolic extract of Mich...

A convenient way to estimate the number of viable cells growing in microtitre tray wells is to use a colorimetric assay and an automatic microplate scanning spectrophotometer. One such assay, developed by Mosmann, depends on the reduction by living cells of tetrazolium salt, MTT, to form a blue formazan product. However the original technique has several technical limitations, namely a less tha...

Early myocardial infarction is usually detected (3) The effect of storage and drying on the timgrossly by utilising the nitro-blue tetrazolium (NBT) ing of the differentiation step was also technique.1 The normal areas stain dark blue and studied. infarcted areas which lack the dehydrogenase enzyme remain unstained. In 1971, Lie et aI2 described a non-enzymatic histochemical method, the haemato...