Breslow (1) has formulated a mechanism of thiamine action in which the Z-position of the thiazolium ring of thiamine is postulated to be the reactive part of the molecule. Krampitz et al. (2) have prepared thiamine with an cr-hydroxyethyl group substituted on the Z-position and reported its probable identity as “active acetaldehyde.” Recent results from thii laboratory indicate that the pyropho...

Thiamine stimulates the production of a red pigment, which is chromatographically and spectrophotometrically identical to prodigiosin, by growing cultures of Serratia marcescens mutant 9-3-3. This mutant is blocked in the formation of 2-methyl-3-amylpyrrole (MAP), the monopyrrole moiety of prodigiosin, but accumulates 4-methoxy-2,2,'-bipyrrole-5-carboxaldehyde (MBC) and can couple this compound...

The electrochemical behavior of thiamine on a self-assembled electrode of L-cysteine (Cys/SAM/Au) has been investigated and Cys/SAM/Au can be used to detect thiamine using square-wave voltammetry (SWV). At pH 11.40 Britton-Robinson buffer, thiamine exhibits a well-defined anodic peak on Cys/SAM/Au. Under the optimized conditions, the anodic peak current of SWV was linear with the content of thi...

Since cocarboxylase, derived from thiamine, is necessary for normal utilization of pyruvate by heart muscle, the importance of adequate body stores of thiamine in patients with chronic heart disease is quite obvious. In order to investigate the occurrence of subclinical thiamine deficiency in patients with chronic heart disease, the thiamine was determined in the four-hour specimen of urine aft...

Increased renal clearance of thiamine (vitamin B(1)) occurs in experimental and clinical diabetes producing thiamine insufficiency mediated by impaired tubular re-uptake and linked to the development of diabetic nephropathy. We studied the mechanism of impaired renal re-uptake of thiamine in diabetes. Expression of thiamine transporter proteins THTR-1 and THTR-2 in normal human kidney sections ...

In their recent systematic review, ter Borg et al. estimated that 50% of older (≥65 years) men and 39% of older women are failing to reach the estimated average requirement for thiamine. This is noteworthy, as thiamine has a unique role in brain physiology as an essential cofactor for glucose metabolism; it is especially important for normal cognitive function in the elderly; and thiamine insuf...

Mutants lacking the first enzyme in de novo purine synthesis (PurF) can synthesize thiamine if increased levels of pantothenate are present in the culture medium (J. L. Enos-Berlage and D. M. Downs, J. Bacteriol. 178:1476-1479, 1996). Derivatives of purF mutants that no longer required pantothenate for thiamine-independent growth were isolated. Analysis of these mutants demonstrated that they w...

To the Editor: Thiamine responsive megaloblastic anemia (TRMA) syndrome is caused by the deficiency of thiamine transporter protein is a triad of diabetes mellitus, anemia, and deafness [1]. The only gene known to be associated with TRMA is SLC19A2, which encodes the high-affinity thiamine transporter [2]. A total of 28 mutations in the SLC19A2 gene have been reported in 70 patients [3]. We rep...

Since cocarboxylase, derived from thiamine, is necessary for normal utilization of pyruvate by heart muscle, the importance of adequate body stores of thiamine in patients with chronic heart disease is quite obvious. In order to investigate the occurrence of subclinical thiamine deficiency in patients with chronic heart disease, the thiamine was determined in the four-hour specimen of urine aft...

Since cocarboxylase, derived from thiamine, is necessary for normal utilization of pyruvate by heart muscle, the importance of adequate body stores of thiamine in patients with chronic heart disease is quite obvious. In order to investigate the occurrence of subclinical thiamine deficiency in patients with chronic heart disease, the thiamine was determined in the four-hour specimen of urine aft...