Understanding fate of antibiotic resistant bacteria (ARB) vs. their antibiotic resistance genes (ARGs) during wastewater sludge treatment is critical in order to reduce the spread of antibiotic resistance through process optimization. Here, we spiked high concentrations of tetracycline-resistant bacteria, isolated from mesophilic (Iso M1-1-a Pseudomonas sp.) and thermophilic (Iso T10-a Bacillus...

Thermophilic viruses represent a novel source of genetic material and enzymes with great potential for use in biotechnology. We have isolated a number of thermophilic viruses from geothermal areas in Iceland, and by combining high throughput genome sequencing and state of the art bioinformatics we have identified a number of genes with potential use in biotechnology. We have also demonstrated t...

alpha-Pinene biodegradation was evaluated in mesophilic and thermophilic biotrickling filters. The potential of silicone oil for enhancing the removal was evaluated too, at both temperatures. Performance was studied at empty bed residence times between 60 and 14s, and concentrations of 0.06-38.84 gm(-3), with or without silicone oil. Efficiency decreased as the pollutant concentration was incre...

Recent years in prokaryotic ribosome research may be characterized by major conceptual revisions, coupled with attempts at resolving ambiguities (e.g. [1,2,3’,4*]) and the introduction of substantial spatial rearrangements [ 51. Thus, the ribosomal functions can no longer be attributed solely to the ribosomal proteins [6**], and the once favorable research object, the Escbe&biu coli ribosome, i...

: Extremophiles are organisms that can grow and thrive in harsh conditions, e.g., extremes of temperature, pH, salinity, radiation, pressure and oxygen tension. Thermophilic, halophilic and radiation-resistant organisms are all microbes, some of which are able to withstand multiple extremes. Psychrophiles, or cold-loving organisms, include not only microbes, but fish that live in polar waters a...

Background: Proteins of various compositions are required by organisms inhabiting different environments. The energetic demands for protein formation are a function of the compositions of proteins as well as geochemical variables including temperature, pressure, oxygen fugacity and pH. The purpose of this study was to explore the dependence of metastable equilibrium states of protein systems on...

Anaerobic digestion (AD) is one of the few sustainable technologies that both produce energy and treat waste streams. Driven by a complex and diverse community of microbes, AD may be affected by different factors, many of which also influence the composition and activity of the microbial community. In this study, the biodiversity of microbial populations in innovative mesophilic/thermophilic te...

Thermophilic organisms require proteins that maintain their structure and activity in the extreme temperature conditions in which their hosts thrive. In view of evaluating the influence of temperature on the different types of interactions that stabilize protein structures, we developed a new approach based on temperature-dependent mean force potentials. Our results show that the stabilizing we...

BACKGROUND Separating acidification and methanogenic steps in anaerobic digestion processes can help to optimize the process and contribute to producing valuable sub-products such as methane, hydrogen and organic acids. However, the full potential of this technology has not been fully explored yet. To assess the underlying fermentation process in more detail, a combination of high-throughput se...