1. A highly stable ATPase (TF,) was purified to a monodispersed state from the membranes of a thermophilic bacterium PS3. Its molecular weight was 380,000, and it was composed of five subunits cy, p, y, a’, and 6 with molecular weights of 56,000, 53,000, 32,000, 15,500, and 11,000, respectively. 2. TF, was stable against dissociating agents such as 5.5 M urea and 4.0 M LiCl, organic solvents, s...

A moderately thermophilic, facultatively anaerobic, amylolytic bacterium was isolated from palm wine, a tropical alcoholic beverage that was sampled in Senegal. The cells were gram positive, catalase positive, non-spore forming, rod shaped, and slightly motile with peritrichous flagella. The strain which we examined did not possess cytochrome and produced L-(+)-lactate, acetate, ethanol, and fo...

The thermophilic, anaerobic, propionate-oxidizing bacterial populations present in the methanogenic granular sludge in a thermophilic (55 degrees C) upflow anaerobic sludge blanket reactor were studied by cultivation and in situ hybridization analysis. For isolation of propionate-degrading microbes, primary enrichment was made with propionate as the sole energy source at 55 degrees C. After sev...

Virtually all members of the order Thermotogales have demonstrated the ability to produce hydrogen and some members of this order produce considerably greater quantities than the rest. With one representative of this order, Thermotoga neapolitana, we have consistently obtained accumulations of 25-30% hydrogen with carbon dioxide as the only other prominent byproduct of the batch reaction (12-15...

Thermal bacteria that live in higher temperature have been considered as good candidates for bioremediation and processing of protein-rich wastewater. However, very little is known about the proteases, the enzymes that digest the protein wastes in these organisms. In this study, we present a comparative genomic analysis of the protease complement in a thermal bacterium Coprothermobacter proteol...

A moderately thermophilic Gram-positive chemo-organotrophic bacterium, strain SP2, was isolated by serial dilutions with crotonate and yeast extract as substrates from a butyrate-degrading methanogenic enrichment obtained thermophilically digested sludge of the Kuryanoskaya wastewater treatment plant (Moscow, Russia). Cells SP2 are spore-forming rods, sometimes occurring in short chains. The ba...