Vibrio parahaemolyticus ( Vp ), a typical microorganism inhabiting marine ecosystems, uses pathogenic virulence molecules such as hemolysins to cause bacterial infections of both human and animals. The thermolabile hemolysin TLH lyses erythrocytes by phospholipase B/A2 enzymatic activity in egg-yolk lecithin. However, few studies have been characterized the biochemical properties use molecular ...

The article analyzes the state of problem manufacturing fast food products from combined fish and vegetable raw materials. properties main auxiliary types materials included in prescription composition snacks are described, chemical manufactured is determined. There considered methods improving design a single-screw extruder order to reduce negative thermal impact on processed thermolabile obta...

Photocatalytic properties of semiconductor materials, studied now for decades, represent huge potentialities as environmentally friendly self-cleaning surfaces. Up to now, inexpensive and non-toxic TiO2-based photocatalysts remain the most interesting solution from practical industrial point view. Nevertheless, obtaining titania surface in its anatase phase, polymorph presenting best photocatal...

BACKGROUND Methylenetetrahydrofolate reductase (MTHFR; EC 1.7.99.5) supplies the folate needed for the metabolism of homocysteine. A reduction in MTHFR activity, as occurs in the homozygous state for the 677C-->T (so-called thermolabile) enzyme variant (TT genotype), is associated with an increase in plasma total homocysteine (tHcy). OBJECTIVE In vitro studies suggest that the reduced activit...

Adenylosuccinate lyase (ADSL) deficiency (MIM 103050) is an autosomal recessive inborn error of purine synthesis characterized by the accumulation in body fluids of succinylaminoimidazolecarboxamide (SAICA) riboside and succinyladenosine (S-Ado), the dephosphorylated derivatives of the two substrates of the enzyme. Because ADSL-deficient patients display widely variable degrees of psychomotor r...

BACKGROUND Alkaline phosphatase (AP) catalyzes the hydrolytic cleavage of phosphate monoesters under alkaline conditions and plays important roles in microbial ecology and molecular biology applications. Here, we report on the first isolation and biochemical characterization of a thermolabile AP from a metagenome. RESULTS The gene encoding a novel AP was isolated from a metagenomic library co...