The method of evaluating cold injury in woody plants by the use of triphenyl tetrazolium chloride has been refined to eliminate bias associated with visual differentiation between varying degrees of tetrazolium reduction and to predict tissue survival at a later date. An advantage of the method described here is that a smuall amount of tissue (50-100 mg) is required; this, therefore, allows !fo...

Abstract The energy profiles for ring opening of representative type B mesoionic 2,3-diphenyl-1,2,3,4-tetrazolium-5-olates, -thiolates, -aminides and –methylides alternative recyclisation pathways are investigated using ab initio MP2 calculations. energetics initial found to be comparable all systems, but the tetrazolium-5-olates anomalous in that no reaction pathway is accessible. influence so...

Inrat liver microsomal fractions oxidation of adrenaline was effected by superoxide anion radicals (A), whereas cytochrome c, 2,6-dichlorophenol-indophenol and ferricyanide accepted electrons from NADPH-specific filavoprotein only directly. Nitro Blue Tetrazolium was reduced both by 02and by tbe direct acceptance of electrons. Elevation ofpH and addition of menadione shift the Nitro Blue Tetraz...

BACKGROUND Different parts of Podocarpus henkelii have been used in many cultures around the world to treat ailments such as cholera, stomach diseases, rheumatism, cancer, canine distemper in dogs and gall sickness in cattle. The aim of this study was to evaluate the biological activity and toxicity of isolated compounds from Podocarpus henkelii after an earlier study indicated a promising acti...

Studies with rat brain illustrate the usefulness of formol-calcium-fixed tissue for studying both enzymatic "chemoarchitectonics" and intracellular organelles. Unembedded frozen sections and polyvinyl alcohol-embedded sections may be used to demonstrate the activities of DPNH-tetrazolium reductase localized in mitochondria and ergastoplasm, TPNH-tetrazolium reductase localized in mitochondria, ...