OBJECTIVE To determine if there are areas of major and minor perfusion in a single testicle, and if the quality and quantity of sperm are correlated with the level of perfusion, we collected testicular tissue from areas with different levels of perfusion. DESIGN Controlled clinical study. SETTING Consecutive patients with azoospermia. PATIENT(S) Patients with azoospermia undergoing testic...

BACKGROUND We performed this study to evaluate use of fresh and frozen sperm samples in non-obstructive azoospermia microdissection testicular sperm extraction (micro-TESE-ICSI) treatment. METHODS We performed a total of 82 consecutive in vitro fertilization (IVF) cycles at Fertijin IVF Center in Istanbul, Turkey from January 2010 to March 2012. In 43 participants we used fresh sperm and froz...

Testicular biopsy was considered the cornerstone of male infertility diagnosis for many years in men with unexplained infertility and azoospermia. Recent guidelines for male infertility have limited the indications for a diagnostic testicular biopsy to the confirmation of obstructive azoospermia in men with normal size testes and normal reproductive hormones. Nowadays, testicular biopsies are m...

BACKGROUND Extensive research is ongoing to empower cancer survivors to have biological parenthood. For this, sperm are cryopreserved prior to therapy and in younger children testicular biopsies are cryopreserved with a hope to mature the germ cells into sperm later on for assisted reproduction. In addition, lot of hope was bestowed on pluripotent embryonic and induced pluripotent stem cells to...

OBJECTIVE To evaluate the impact of sperm defect severity and the type of azoospermia on the outcomes of intracytoplasmic sperm injection (ICSI). MATERIALS AND METHODS This study included 313 ICSI cycles that were divided into two major groups according to the source of spermatozoa used for ICSI: 1) Ejaculated (group 1; n = 220) and 2) Testicular/Epididymal (group 2; n = 93). Group 1 was subd...

Effect of a combination of pesticides, viz. Delatmethrin 1% + Chlorpyriphos 35% EC, has been studied on the reproductive toxicology of wistar rats. Male rats were exposed to test substance at the dose level of 15, 25, and 35-mg/kg body weight for a consecutive period of 20 weeks. At the end of the experimental period, all the animals were subjected for necropsy, sperm parameters such as motilit...

Based on histological investigation of the testes in 270 patients with functional infertility, in 13 patients with stenotic lesion and in 11 men with proven fertility, the following conclusions were obtained. 1) Testicular biopsy should be of first choice in the examination of male infertility. Epididymo-vaso-vesiculography to prove the patency of the seminal tracts is not always necessary. It ...

background: stress is a cause of male infertility. although sex hormones and sperm quality have been shown to be low in stress, sperm physiology and testicular functional proteins, such as phosphotyrosine proteins, have not been documented. objective: to investigate the acrosome status and alterations of testicular proteins involved in spermatogenesis and testosterone synthesis in chronic stres...

AIM To evaluate the outcome of repetitive micro-surgical testicular sperm extraction (mTESE) attempts in non-obstructive azoospermia (NOA) cases, in relation to patients' initial testicular histology results. METHODS A total of 68 patients with NOA in whom mTESE had been performed in previous intracytoplasmic sperm injection (ICSI) attempts were reviewed. RESULTS Among the 68 patients with ...

Bisphenol A (BPA) is an estrogenic environmental toxicant, a synthetic monomer widely used to polymerize polycarbonate plastics and resins. BPA has been implicated to have hazardous effect on reproductive health in human and experimental rats. Lycopene is a powerful antioxidant and potential high-value compound for the prevention of many diseases. The present study has been designed to investig...