Virus mutants (NDV(pi)) recovered from L cells persistently infected with Newcastle disease virus (NDV, Herts strain) are temperature-sensitive (ts) at 43 C, although the wild-type virus (NDV(o)) which initiated the persistent infection replicates normally at that temperature. To study the relationship between the ts marker of NDV(pi) and the other properties which distinguish this virus from N...

Methods to increase temperature stability of vaccines and adjuvants are needed to reduce dependence on cold chain storage. We report herein creation and application of pVEX expression vectors to improve vaccine and adjuvant manufacture and thermostability. Defined media fermentation yields of 6g/L thermostable toll-like receptor 5 agonist flagellin were obtained using an IPTG inducible pVEX-fla...

CONTEXT Levothyroxine monotherapy is the treatment of choice for hypothyroid patients because peripheral T4 to T3 conversion is believed to account for the overall tissue requirement for thyroid hormones. However, there are indirect evidences that this may not be the case in all patients. OBJECTIVE To evaluate in a large series of athyreotic patients whether levothyroxine monotherapy can norm...

Cold agglutinins are of unique relevance in cardiac surgerybecause of the use of hypothermic cardiopulmonary bypass (CPB). Cold autoimmune diseases are defined by the presence of abnormal circulating proteins (usually IgM or IgA antibodies) that agglutinate in response to a decrease in body temperature. These disorders include cryoglobulinemia and cold hemagglutinin disease.Immunoglobulin M aut...

We have constructed and expressed a series of mutant influenza virus hemagglutinins, each containing a new consensus site for glycosylation in addition to the seven sites found on the wild-type protein. Oligosaccharide side chains were added with high efficiency at four of the five novel sites, located on areas of the protein's surface that are not normally shielded by carbohydrate. Investigati...

We developed an influenza hemagglutinin (HA) pseudotype library encompassing Influenza A subtypes HA1-18 and B (both lineages) to be employed in microneutralization (pMN) assays. The pMN is highly sensitive specific for detecting virus-specific neutralizing antibodies against viruses can used assess antibody functionality vitro. Here we show the production of these viral HA pseudotypes their em...

Abstract Background Influenza viruses are a continuous threat to avian and mammalian species, causing epidemics pandemics. After the circulation of H5N1 in 2006, 2015, 2016 Iraq, an H5N8 influenza virus emerged domestic geese Sulaymaniyah Province, Iraq. This study analyzed genetic characteristics Iraqi viruses. Results An HPAI subtype was identified from backyard Kurdistan Region, north Phylog...