Effective cell migration, which plays an essential role in many physiological events, including tissue morphogenesis, wound healing and inflammatory responses, is dependent on dynamic remodeling of the actin-cytoskeleton. Type I coronins, a conserved class of WD40-repeat proteins, have been shown to facilitate this process by modulating the turnover of branch-actin networks at the leading edge....

Transmission electron microscopy (TEM) and epifluorescence microscopy were used to obtain comparative measurements of total bacterial counts, and to enumerate abundances of various bacterial morphotypes in an eutrophic freshwater habitat. Although particulate matter would have been expected to interfere with counting by obscuring large areas of the electron microscope grids, estimates of total ...

To determine the prevalence and genotypes of extended-spectrum beta-lactamases (ESBLs) among clinical isolates of Klebsiella pneumoniae and Escherichia coli, we performed antibiotic susceptibility testing, pI determination, induction testing, transconjugation, and DNA sequencing analysis. Among the 509 isolates collected from 13 university hospitals in Korea, 39.2% produced ESBLs. ESBL-producin...

Advances in vacuum-tight in situ liquid cell TEM systems have attracted significant attention because of the ability to directly interpret chemical and electrochemical reactions within their native liquid environments [1]. Conducting quantitative in situ electrochemistry experiments within the S/TEM is feasible using the relatively new platform of in situ electrochemical S/TEM (ec-S/TEM). In th...

The interaction of cells with their supporting extracellular matrix influences cellular phenotype, cellular proliferation, protein synthetic profile, and specific gene activation. To examine the ability of culture substratum to modulate the phenotype expressed by glomerular epithelial cells (GEC) in culture, GEC were grown on plastic culture plates coated with collagen gels (Type I collagen, Vi...

background: gold nanoparticles (gnps) are used in imaging and molecular diagnostic applications. as the development of a novel approach in the green synthesis of metal nanoparticles is of great importance and a necessity, a simple and safe method for the synthesis of gnps using plant extracts of zataria multiflora leaves was applied in this study and the results on gnps’ anticancer activity aga...

The Purkinje cell and their synaptic contacts have been described using (1) light microsocopy, (2) transmission and scanning electron microscopy, and freeze etching technique, (3) conventional and field emission scanning electron microscopy and cryofracture methods, (4) confocal laser scanning microscopy using intravital stain FM64, and (5) immunocytochemical techniques for Synapsin-I, PSD9-5, ...

cells play a pivotal role in the pathogenesis of autoimmunity (4). Wulff et al. described that the characteristic potassium channel phenotype of TEM cells in multiple sclerosis (MS) is Kv1.3high IKCa1low, contrasting naïve, and central memory T (TCM) cells, which exhibit a Kv1.3low IKCa1high channel phenotype (1). Therefore the therapeutic relevance of specific Kv1.3 channel inhibitors is of ou...

In this article, we report on the use of transmission electron microscopy (TEM) for the fabrication of high-performance textured amorphous/crystalline silicon (a-Si:H/c-Si) heterojunction (HJ) solar cells. Whereas classical thin-film characterization techniques allowed us to optimize the a-Si:H layer properties for flat HJ solar cells (open-circuit voltages (VOC) up to 710 mV and energy convers...