Recent development of liquid phase transmission electron microscopy (TEM) enables the study of specimens in wet ambient conditions within a liquid cell; however, direct structural observation of biological samples in their native solution using TEM is challenging since low-mass biomaterials embedded in a thick liquid layer of the host cell demonstrate low contrast. Furthermore, the integrity of...

The crystal phase of Au-NWs is assessed by means of high-resolution transmission electron microscopy (HR-TEM). NWs are dispersed on a transmission electron microscopy (TEM) grid and imaged with a JEOL 4000EX-II TEM. Figure S-2(a) shows a HR-TEM lattice imaging of a representative Au-NW. The measured interplanar spacing (3.74 Å) is consistent with the (110) planes of a CdSe crystal with the wurt...

The feasibility of using a focused ion beam (FIB) for the purpose of thinning vitreously frozen biological specimens for transmission electron microscopy (TEM) was explored. A concern was whether heat transfer beyond the direct ion interaction layer might devitrify the ice. To test this possibility, we milled vitreously frozen water on a standard TEM grid with a 30-keV Ga(+) beam, and cryo-tran...

During inflammation, T lymphocytes migrate out of the blood across the vascular endothelium in a multistep process. The receptors mediating T cell adhesion to endothelium are well characterized; however, the molecules involved in T cell transendothelial migration (TEM) subsequent to lymphocyte adhesion to the endothelium are less clear. To identify receptors mediating TEM, mAbs were produced ag...

Ceftazidime-resistant Escherichia coli and Klebsiella pneumoniae (49 and 102 isolates, respectively) were collected from Barnes-Jewish Hospital, St. Louis, Mo., from 1992 to 1996. They were uniformly resistant to ceftazidime, generally resistant to aztreonam, and variably susceptible to cefotaxime. Four representative E. coli strains and 15 Klebsiella strains were examined. From one to four bet...

Nano is one of the hottest topics in current science and technology. Characterizations of nanomaterials by high resolution transmission electron microscopy (TEM) are becoming indispensable today. To gain better performance of TEM, people are expecting a novel TEM grid of which the supporting film should be highly conductive, ultrathin but robust, and preferably nanoholey. Here we report a metho...

We use the occasion of a change in tax policy that raised the tax rate for many of the listed companies in China to examine tax-induced earnings management (TEM) from the perspective of political connections. We find that when the tax rate increased, only those affected firms with politically connected management engaged in TEM. This suggests that, in addition to motivation for managing earning...