نتایج جستجو برای: taqman real time pcr

تعداد نتایج: 2269818  

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه ارومیه - دانشکده علوم پایه 1393

ما در این کار به مطالعه ی اثر داروی ماینوکسیدل و لیزات پلاکتی انسان بر روی میزان بیان ژن های ناحیه ی بالب و بالج فولیکول موی موش سوری با استفاده از روش real time-pcr و rt-pcr پرداختیم.

2007
Juliet P. Dukes Donald P. King Soren Alexandersen

Loop-mediated amplification (LAMP) amplifies specific nucleotide sequences but unlike PCR, it runs at a single temperature and positive reactions can be visualized with the naked eye so fragile instrumentation is not required. We have developed a one step, single-tube, accelerated, FMDVspecific RT-LAMP assay for the rapid detection of all seven serotypes of the virus, using primers designed aga...

Journal: :BMC Biotechnology 2008
Meti Buh Gašparič Katarina Cankar Jana Žel Kristina Gruden

BACKGROUND The real-time polymerase chain reaction is currently the method of choice for quantifying nucleic acids in different DNA based quantification applications. It is widely used also for detecting and quantifying genetically modified components in food and feed, predominantly employing TaqMan and SYBR Green real-time PCR chemistries. In our study four alternative chemistries: Lux, Plexor...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه زابل - دانشکده کشاورزی 1388

پژمردگی ورتیسلیومی یکی از بیماری های مهم زیتون است که باعث تخریب سیستم آوندی و در نهایت مرگ گیاه می شود. استفاده از ارقام مقاوم یکی از موثرترین روش های کنترل بیماری محسوب می شود. دراین بررسی ابتدا از زیتون های آلوده منطقه طارم در استان زنجان نمونه برداری صورت گرفت. پس از ضدعفونی سطحی، قطعات نمونه برداری شده بر روی محیط کشت های عمومی pdaو اختصاصی czapec dox agar دردمایc 24ْ و شرایط تاریکی در انکو...

2002
Renato Biondo

We have developed a real-time quantitative PCR assay to measure the concentration of DNA extracted in forensic biological traces. Its rapid and sensitive results were obtained in less than 4 hours. This real-time quantification can be performed on the ABI Prism 7700 Sequence Detector using the fluorogenic probe in a TaqMan assay. We suggest a protocol that will be able to detect and quantitate ...

Journal: :BioTechniques 2008

Journal: :Journal of clinical microbiology 2006
Jens Dreier Melanie Störmer Dietrich Mäde Sabine Burkhardt Knut Kleesiek

We have developed a one-tube reverse transcription (RT)-PCR method using the real-time TaqMan PCR system for the detection of norovirus genogroup I (NV GGI). By introduction of a novel probe based on locked nucleic acid technology, we enhanced the sensitivity of the assay compared to those of conventional TaqMan probes. The sensitivity of the NV GGI RT-PCR was determined by probit analysis with...

2013
nursen topcuoglu ceki Paltura Mehmet Kulekci Guven Kulekci

Oral and middle ear infections are biofilm related infections. Fusobacterium nucleatum is considered to be a key species in dental biofilm formation. The aim of this study was to compare conventional and real-time PCR, for the detection of F. nucleatum in saliva, nasopharyngeal secretion and middle ear effusion samples. Sixty samples were collected from 20 patients with otitis media with effusi...

Journal: :Journal of clinical microbiology 2000
C E Corless M Guiver R Borrow V Edwards-Jones E B Kaczmarski A J Fox

A set of universal oligonucleotide primers specific for the conserved regions of the eubacterial 16S rRNA gene was designed for use with the real-time PCR Applied Biosystems 7700 (TaqMan) system. During the development of this PCR, problems were noted with the use of this gene as an amplification target. Contamination of reagents with bacterial DNA was a major problem exacerbated by the highly ...

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