The incidences of congenital toxoplasmosis in Fischer rats infected between the 8th and 12th days of pregnancy with three different strains of Toxoplasma gondii (RH, 76K, and Prugniaud) were 58.2, 35.2, and 62.8%, respectively. No infected fetuses were collected from rats previously infected with RH or Prugniaud strain parasites, even if the rats were reinfected during pregnancy. Since pups fro...

The various infection routes of Toxoplasma gondii that are close to daily life strongly support the incidence toxoplasmosis. emergence drug-resistant strains raises future concerns. Moringa leaf ethanol extract has been shown have several anti-pathogen activities, which could an anti-Toxoplasma effect. This research was conducted analyze effect moringa against tachyzoites replication in and cor...

Phenacoccus solenopsis, the cotton mealy bug, has emerged as a serious pest of cotton in india, necessitating basic studies on its development and survival at different temperatures (20, 25, 30 and 35 °C) and relative humidities (rH) (65, 75 and 85 ± 1 %) levels to ascertain optimal population growth. a life table was constructed using temperature and humidity. The nymphal duration was 20.2 d a...

Acute and chronic Toxoplasma infections were evaluated in mice using stage specific antibodies and immunocytochemistry. Mice with acute toxoplasmosis were less active, had erectile body hair and seldom took food or water resulting in weight loss. All mice died within 7 days post-inoculation. The immunohistochemical technique enhanced visualization of parasites allowing their distribution to be ...

PURPOSE Toxoplasma gondii, the parasite responsible for ocular toxoplasmosis, accesses the retina from the bloodstream. We investigated the dendritic cell as a potential taxi for T. gondii tachyzoites moving across the human retinal endothelium, and examined the participation of adhesion molecules and chemokines in this process. METHODS CD14-positive monocytes were isolated from human periphe...

Stainless steel disks (diameter, 1 cm) were contaminated with fecally suspended hepatitis A virus (HAV; strain HM-175) and held at low (25% +/- 5%), medium (55% +/- 5%), high (80% +/- 5%), or ultrahigh (95% +/- 5%) relative humidity (RH) at an air temperature of 5,20, or 35 degrees C. HAV survival was inversely proportional to the level of RH and temperature, and the half-lives of the virus ran...

Strength, stiffness, strain at fracture and moisture content of onion skins were measured after incubation at a range of humidities. Higher relative humidity (RH) resulted in skins which had greater moisture content, were stronger in multi-directional tests and which were less stiff. In uni-directional tests, skins incubated at higher RH had extended more at fracture than those incubated at low...

To eliminate apicomplexan parasites, inhibitory compounds must cross host cell, parasitophorous vacuole, and parasite membranes and cyst walls, making delivery challenging. Here, we show that short oligomers of arginine enter Toxoplasma gondii tachyzoites and encysted bradyzoites. Triclosan, which inhibits enoyl-ACP reductase (ENR), conjugated to arginine oligomers enters extracellular tachyzoi...

Titers in a direct agglutination test using Formalin-fixed toxoplasma tachyzoites were significantly higher in patients with acquired immunodeficiency syndrome with toxoplasmic encephalitis (TE) than in those without TE. In contrast, when fresh (not fixed) tachyzoites were used, the titers did not differ from those in patients without TE. The direct agglutination test using Formalin-fixed tachy...

The effect of clindamycin (CLI) combined with autovaquone (ATO) was examined in a murine model of acute toxoplasmosis. Swiss Webster mice intraperitoneally infected with 10(2) or 10(4) tachyzoites of the RH strain of Toxoplasma gondii were perorally treated with either drug alone (for ATO, 5, 25, 50, or 100 mg/kg of body weight/day; for CLI, 25, 50, or 400 mg/kg/day) or both combined (for ATO p...