Background: The poor permeability of the plasma and nuclear membranes to DNA plasmids are two major barriers for the development of these therapeutic molecules. Therefore, success in gene therapy approaches depends on the development of efficient and safe non-viral delivery systems. Objectives: The aim of this study was to investigate the in vitro delivery of plasmid DNA encoding HPV16 E7 gene...

In recent years, the number of recombinant proteins used for therapeutic applications and industry has increased dramatically. Recombinant proteins are produced in many host organisms (microbial, insect, plant and mammalian cells). There are many factors to consider when choosing the optimal system for protein expression and purification including the mass, purity or solubility of the recombina...

Introduction: Overexpression of Human Epidermal Growth factor Receptor-2 (HER-2) is one of the most important prognostic and predictive factors of breast cancer, observed in 25% - 30% of breast carcinoma patients leading to poor prognosis and feasible anti HER-2 antibody drugs. The objective of this study was to evaluate the HER-2 frequency in target population and its correlat...

introduction: colorectal cancer (crc) is one of the most prevalent cancers in the world. genetic disorders and dietary habits play a part in crc. the most important cancer suppressor gene is p53. different mutations rates in 7th exon of p53 gene are reported in different areas  which may have a relationship with prognosis of crc. this study examines the relationship between mutation in 7th exon...

objectives the cause of rheumatoid arthritis (ra) as a chronic inflammatory autoimmune disease is still unknown. it appears that both genetic and environmental factors play a role in its pathogenesis. recent studies reveal that in addition to the cns, immune cells synthesis neurotransmitters so that these catecholamines can regulate immune functions. the aim of this study is to evaluate the dop...