Compatibility between plants and obligate biotrophic fungi requires fungal mechanisms for efficiently obtaining nutrients and counteracting plant defenses under conditions that are expected to induce changes in the host transcriptome. A key step in the proliferation of biotrophic fungi is haustorium differentiation. Here we analyzed global gene expression patterns in Arabidopsis thaliana leaves...

Embryogenic tissues of Abies alba Mill. were cryopreserved using the slow-freezing approach. Four cell lines incubated for 24 h on a medium with 0.5 M sorbitol and pre-treated 5% DMSO. Subsequently, frozen at cooling rate 1 °C min-1 to -40 transferred liquid nitrogen 72 hours. After thawing in water bath 40 °C, cultivated proliferation medium. All tested recovered, but variations regrowth frequ...

In the context of a symbiotic plant-fungus interaction study concerning Cattleya purpurata, we focused on some aspects seed morphology and biology, early stages seedling development. Seed was characterized using light scanning electron microscopy. vitro germination capability evaluated, comparing asymbiotic methods. The seeds overall comparable to that other congeneric species, showing classica...

In contrast to animals, the basic body plan of plants develops largely postembryonically and is directed by two primary meristems located on opposite ends of a bipolar embryo (Jürgens, 2001). The basal root meristem serves to extend the primary root established in the embryo, whereas the apical shoot meristem both maintains growth and provides a source of cells for new organs such as leaves and...

Stripe rust caused by the fungus Puccinia striiformis f.sp. tritici (Pst) is a major constraint to wheat production worldwide. The molecular events that underlie Pst pathogenicity are largely unknown. Like all rusts, Pst creates a specialized cellular structure within host cells called the haustorium to obtain nutrients from wheat, and to secrete pathogenicity factors called effector proteins. ...