Theoretical considerations illustrate the feasibility and characteristics of continuous virus cultivation in cell suspensions Physical dimensions of the virus fermentor (lysostat) in relation to optimal yields can be predetermined. Poliovirus type 1 was grown on Hela S-3-1 (Saltzman) cells in a single-stage, percolator-type lysostat, with a yield of 421 TCID(50) per cell. Adenovirus type 14 was...

there are many evident that people are more interested in walking in attractive urban spaces and inappropriate physical and psychological condition can reduce their presence in a remarkable amount. the current research investigates the influence of physical design and psychological elements and their criteria on selective physical activity and specifically walking in urban public spaces. howeve...

The present work was aimed to examine the effects of incorporation of each of two different types of nanoclay, i.e. Cloisite Na+ and Cloisite 30B, into PVA foam on cellular morphology and water absorption capacity. Foam samples containing 0.0-10.0 wt% of each of the two types of nanoclay alone were prepared using mechanical foaming. Accordingly, PVA/organoclay/water suspensions were prepare...

Analysis of electron spin resonance spectra of 5-doxyl stearic acid in aqueous suspensions of Clostridium acetobutylicum ATCC 824 and the butanol-tolerant SA-2 derivative during a small-scale fermentation at three different butanol challenge levels indicated that the SA-2 strain is able to respond to the physical fluidizing effect of high (1.5%) butanol challenge by reducing its membrane fluidi...

The resistance of single cell suspensions to osmotic and ther mal injury has been determined by use of vital dye exclusion to document the integrity of the cell surface. Four newly derived leukemias possessed a resistance to injury by these physical agents that was similar to that of normal marrow cells, but higher than that of various types of normal lymphocytes. On continued isotransplantatio...