SCOPE Sulforaphane (SF) is a natural isothiocyanate in broccoli sprouts with cancer chemopreventive activity. This study is aimed to use different methods to develop broccoli sprout preparations to compare their ability to deliver SF to the mice and to evaluate the kinetics and biodistribution of SF. METHODS AND RESULTS The SF-enriched sprout preparation generated by two-step procedure (quick...

Effect of pre-harvest methyl jasmonate (MeJA) and post-harvest 1-methylcyclopropene (1-MCP) treatments on broccoli floret glucosinolate (GS) concentrations and quinone reductase (QR, an in vitro anti-cancer biomarker) inducing activity were evaluated two days prior to harvest, at harvest and at 10, 20, and 30 days of post-harvest storage at 4 °C. MeJA treatments four days prior to harvest of br...

Neurons rely on the release and subsequent cleavage of GSH to cysteinylglycine (CysGly) by astrocytes in order to maintain optimal intracellular GSH levels. In neurodegenerative diseases characterised by oxidative stress, neurons need an optimal GSH supply to defend themselves against free radicals released from activated microglia and astroglia. The rate of GSH synthesis is controlled largely ...

On the basis of animal studies, the chemopreventive activity of isothiocyanates has been linked to their ability to modulate carcinogen-metabolising enzyme systems, including cytochrome P450. However, the potential of isothiocyanates to influence these enzyme systems in human liver has not been investigated. We have evaluated the modulation of cytochrome P450 expression in two human liver sampl...

This study aimed to determine the effect of BAP on growth hybrid broccoli and synthesis sulforaphane content grown MS media. Observations were made for 2 weeks, namely first day after planting until 14th planting. used a completely randomized design with 4 treatments was repeated 9 times. The results showed that had germinated. It found highest Broccoli sprout height in treatment without (Contr...

In this issue, Lin et a!. ( 1) show convincing statistics that subjects exposed to dietary broccoli who have a GSTM 12 null phenotype are less susceptible to colon cancer than those who are GSTMI positive. The expected effect of a GSTM1 null phenotype is greater, not less, susceptibility to cancers due to reduced carcinogen detoxification by GSTM1 (2, 3). Broccoli reverses this outcome for two ...

INTRODUCTION Rheumatoid arthritis (RA) is characterized by progressive inflammation associated with rampantly proliferating synoviocytes and joint destruction due to oxidative stress. Recently, we described nuclear factor erythroid 2-related factor 2 (Nrf2) as a major requirement for limiting cartilage destruction. NF-κB and AP-1 are the main transcription factors triggering the inflammatory pr...

Glioblastoma is the most common primary tumor of the brain and has few long-term survivors. The local and systemic immunosuppressive environment created by glioblastoma allows it to evade immunosurveillance. Myeloid-derived suppressor cells (MDSCs) are a critical component of this immunosuppression. Understanding mechanisms of MDSC formation and function are key to developing effective immunoth...