Introduction: Production of a novel polygalacturonase (PG) active at pH = 3.0 and suitable to be used in fruit juice industries from Macrophomina phaseolina was evaluated. Suitable carbon, nitrogen and phosphorous forms were determined and the condition was optimized for higher PG production. Materials and methods: Macrophomina phaseolina was cultured in so called production medium. The secreto...

BACKGROUND Patch testing is an essential procedure in the investigation of eczema in children. OBJECTIVES To analyse the frequency of contact hypersensitivity and allergic contact dermatitis among Polish children with eczema. PATIENTS/METHODS During an allergy screening programme involving 9320 children aged 7 and 16 years, 12.6% reported symptoms of chronic/recurrent eczema. From this grou...

Several prophylactic human papillomavirus (HPV) vaccines have been developed based on virus-like particles (VLPs) made from viral L1 proteins. A substantial number of VLPs is necessary for biochemical characterization and diagnostic test development. To establish the optimum conditions for production and purification of HPV L1 in the yeast expression system we varied the amount and nature of th...

Serum is generally considered to contain at least two proteins, albumin and globulin, differing both in their isoelectric points, (literature summarized by Freundlich) and molecular weights (Svedberg and Sj6gren), and readily separable by half-saturation with ammonium sulfate, which precipitates the globulin. There has been considerable investigation tending to show that the globulin fraction i...

Phosphogypsum (CaSO4·2H2O, PG) waste is produced in large amounts during phosphoric acid (H3PO4) production. Minor quantities are utilized in construction or agriculture, while most of the material is stockpiled, creating an environmental challenge to prevent pollution of natural waters. In principle, the gypsum waste could be used to capture several hundred megatonnes of carbon dioxide (CO2). ...

Cardiac myosin prepared by any one of a number of modifications of the basic Szent-Gyorgyi method and cardiac myosin prepared by the lithium chloride-ammonium sulfate technique differ in two important respects: 1) SzentGyorgyi-prepared myosin solutions are inhomogeneous by both chemical and immunologic criteria; 2) the ATPase activity of Szent-Gyorgyi-prepared myosin is low in comparison to lit...

Agricultural soils can contain high arsenic (As) concentrations due to specific geological contexts or pollution. Fertilizer amendments could influence As speciation and mobility thus increasing its transfer crops toxicity. In the present study, field-relevant amounts of fertilizers were applied from a cultivated field that was former ammunition-burning site. Potassium phosphate (KP), ammonium ...

Background: Erythropoietin, as a principal hormone promotes red blood cell production in bone marrow. Varieties of erythropoietin biosimilar are being produced by recombinant DNA technology in cell cultures. The detection or quantification of these molecules are being performed by diff erent methods which some of theme such as Western blot and enzymelinked immunosorbent assay (ELISA) require sp...

Conditions are reported that, for the first time, permit the folding and assembly of active chaperonin, GroEL, following denaturation in 8 m urea. The folding could be achieved by dilution or dialysis, and the best yields required the simultaneous presence of ammonium sulfate and the Mg2+ complexes of ATP or ADP. Ammonium sulfate was the key to this particular protocol, since there was a small ...

The effectiveness of therapeutic monoclonal antibodies (mAbs) is governed not only by their bioactivity, but also by their biophysical properties. Assays for rapidly evaluating the biophysical properties of mAbs are valuable for identifying those most likely to exhibit superior properties such as high solubility, low viscosity and slow serum clearance. Analytical hydrophobic interaction chromat...