A new methodology was developed to study the cell-surface glycoproteins of cultured human skin fibroblasts. This was based on the binding of a variety of biotinyl-lectins to nitrocellulose electrophoretic transfers of total fibroblast lysates after separation in sodium dodecyl sulphate/polyacrylamide gels, followed by reaction with avidin-biotinyl-peroxidase complexes and detection with 3,3'-di...

The addition of O-linked N-acetylglucosamine (GlcNAc) to the major nuclear pore complex glycoprotein p62 was examined. Expression of the rat p62 cDNA in transfected monkey cells was detected using a rat p62-specific antipeptide antiserum and two previously described nuclear pore-specific monoclonal antibodies which require O-linked GlcNAc for binding. Although the p62 cDNA was predicted to enco...

We investigated the effect of wheat germ agglutinin (WGA) on plant resistance to salinity. We showed that treatment of wheat seedlings with exogenous WGA resulted in an increase in cell division and elongation. Incubation of 4-d-old seedlings in 2% NaCl solution for 24 h resulted in appreciable decrease in mitotic activity of meristem cells in roots and growth of whole seedlings. At the same ti...

The importance of early cancer diagnosis has led to development many different diagnostic methods. In this context, the studies investigating presence and amount sugar residues use as indicators in identification cell type have become prominent. present study, sialic acids found on membrane surfaces ER (+) MCF-7 (-) MDA-MB-231 breast lines were labeled using three-dimensional (3D) culture (Sphe...