In the present study, we propose a novel clustering-based method for modeling accurate fuzzy rule-based classification systems. The new method is a combination of a data mapping method, subtractive clustering method and an efficient gradient descent algorithm. A data mapping method considers the intricate geometric relationships that may exist among the data and computes a new representation of...

UNLABELLED A Genomic Target Database (GTD) has been developed having putative genomic drug targets for human bacterial pathogens. The selected pathogens are either drug resistant or vaccines are yet to be developed against them. The drug targets have been identified using subtractive genomics approaches and these are subsequently classified into Drug targets in pathogen specific unique metaboli...

-Studies of chemical ecology of an organism are founded on the isolation and identification of a semiochemical, often comprised of two or more synergistic compounds (each synergist alone has little activity, but presented together they are bioactive). Chromatographic fractionation and bioassay methods of binary splitting, additive combination, and subtractive combination are compared for effici...

Using PCR-select subtractive cDNA hybridization technique, 57 expressed sequence tags (ESTs) were isolated from 240 clones of a modified live Edwardsiella ictaluri vaccinated vs. sham-vaccinated channel catfish anterior kidney subtractive library. The transcription levels of the 57 ESTs in response to E. ictaluri vaccination were then evaluated by quantitative PCR (QPCR). Of the 57 ESTs, 43 wer...

BACKGROUND AND AIMS Recent papers indicated that epigenetic control is involved in transitions in bud dormancy, purportedly controlling gene expression. The present study aimed to identify genes that are differentially expressed in dormant and non-dormant Castanea sativa buds. METHODS Two suppression subtractive hybridization cDNA libraries were constructed to characterize the transcriptomes ...

The widespread and sustained exploitation of the entomopathogen Bacillus thuringiensis (Bt) in the control of insects, pests and disease vectors is threatened by the evolution of resistance. In the present work, the construction of subtractive libraries and analysis of gene function by RNA interference-mediated gene silencing (RNAi) were combined to determine the molecular basis of resistance t...

A method to approximate derivatives of real functions using complex variables which avoids the subtractive cancellation errors inherent in the classical derivative approximations is described. Numerical examples illustrating the power of the approximation are presented.

The cloning of genes expressing proteins that are differentially expressed in the organ microvasculature has the potential to address a variety of problems ranging from the analysis of disease pathogenesis to drug targeting for particular tissues. This study describes a methodology designed to analyze differential protein expression in the brain microvasculature. The method can be applied to ot...