Mutations, designed by analysis of the crystal structures of Rous sarcoma virus (RSV) and human immunodeficiency virus type 1 (HIV-1) protease (PR), were introduced into the substrate binding pocket of RSV PR. The mutations substituted nonconserved residues of RSV PR, located within 10 A of the substrate, for those in structurally equivalent positions of HIV-1 PR. Changes in the activity of pur...

Alpha-N-acetyl galactosaminidase (alpha-NaGalase) has been reported to accumulate in serum of cancer patients and be responsible for deglycosylation of Gc protein, which is a precursor of GcMAF-mediated macrophage activation cascade, finally leading to immunosuppression in advanced cancer patients. We studied the biochemical characterization of alpha-NaGalase from several human tumor cell lines...

Introduction Enzymes are proteins and are present in all living cells, where they facilitate and/or regulate the numerous chemical reactions taking place. Enzymes act by recognizing and binding to its substrate (i.e., the molecule on which it acts) and usually only act on only one (1) specific substrate. Depending on the type of enzyme, it will then break specific bonds in the substrate, releas...

Porcelain enamel is an inorganic-type coating, which applied to metals or glass for both decorative and functional purposes. This coating a silica-based solidified mass obtained by high-temperature firing (temperature can range between 450 1200 °C depending on the substrate). coatings differ from ceramic mainly their structure dilatation coefficient, organic paints inorganic nature of matrix ch...

The production of biogas has many advantages against other biomass based processes for production of energy or fuel. Biogas can be produced from any type of biomass whether it is a pure substrate or if it is a mix of materials like source-sorted municipal organic solid waste. The biogas process can handle substrate with different water content, from wet materials like Sewage sludge to dry mater...

Recognition of and discrimination between potential glyco-substrates is central to the function of galectins. Here we dissect the fundamental parameters responsible for such selectivity by the fungal representative, CGL2. The 2.1 A crystal structure of CGL2 and five substrate complexes reveal that this prototype galectin achieves increased substrate specificity by accommodating substituted olig...

A gas permeable CHA-type aluminophosphate molecular sieve membrane was ionothermally synthesized on a porous d-alumina substrate through the so-called “substrate surface conversion” method. The synthetic conditions for this specific membrane were optimized. The temperature, time and the amounts of H3PO4 and HF in the initial synthesis solution were investigated. X-ray diffraction (XRD), scannin...

In our previous crystallographic studies of human immunodeficiency virus type 1 (HIV-1) protease-substrate complexes, we described a conserved "envelope" that appears to be important for substrate recognition and the selection of drug-resistant mutations. In this study, the complex of HIV-1 protease with the inhibitor RO1 was determined and comparison with the substrate envelope provides a rati...

The dynamics of liquid condensation on a substrate or within a capillary is studied when the wetting film grows via interface-limited growth. We use a phenomenological time-dependent Ginzburg-Landau (TDGL)-type model with long-range substrate potential. Using an order parameter, which does not directly represent the density, we can derive an analytic formula for the interfacial growth velocity ...

The BT4131 gene from the bacterium Bacteroides thetaiotaomicron VPI-5482 has been cloned and overexpressed in Escherichia coli. The protein, a member of the haloalkanoate dehalogenase superfamily (subfamily IIB), was purified to homogeneity, and its X-ray crystal structure was determined to1.9 A resolution using the molecular replacement phasing method. BT4131 was shown by an extensive substrat...