attempts to obtain experimental values for the kinetic parameters of phenanthridine oxidation by guinea pig or rabbit liver aldehyde oxidase using common spectrophotometric methods have not been successful due to a lower limit of detection. in the present study, a new spectrofluorimetric assay in combination with a multivariate calibration method for enzymatic kinetic study of aldehyde oxidase ...

Plant peroxidases are capable of binding phenolic substrates, and it has been possible to crystallize complexes between horseradish peroxidase C (HRP C) and benzhydroxamic acid. The X-ray structures of the binary HRP C:ferulic acid complex and the ternary HRP C:CN(-):ferulic acid complex to 2.0 and 1.45 A resolution, respectively, have also been solved recently. Ferulic acid is a naturally occu...

1. Experiments were designed to establish the relationship between renal gluconeogenesis and sodium transport. Six inhibitors of gluconeogenesis, with different sites of action, were tested in the isolated perfused rat kidney in which renal function, and in particular Na+ reabsorption, was followed. 2. In kidneys from fed or starved rats specific inhibitors of gluconeogenesis neither inhibited ...

Many proteins of the mitochondrial IMS contain conserved cysteines that are oxidized to disulfide bonds during their import. The conserved IMS protein Mia40 is essential for the oxidation and import of these proteins. Mia40 consists of two functional elements: an N-terminal cysteine-proline-cysteine motif conferring substrate oxidation, and a C-terminal hydrophobic pocket for substrate binding....

Metal (Au, Cu)-modified Si nanowires (SiNWs) are superior catalysts for selective oxidation of hydrocarbons, while SiNWs are a powerful substrate support (enhance efficiency and selectivity) for nanocatalysts.

The heart consumes vast amounts of energy, and cardiac energetic impairment is a characteristic feature of several inherited and acquired diseases of heart muscle. In the fasting state, the heart typically generates ≈70% of its energy from β-oxidation of fatty acids, 10% to 30% from oxidation of glucose, and the remainder from ketones, lactate, and amino acids. However, it is a metabolic omnivo...

Abstract Dense, uniform, and well-adhered chromium (Cr) coatings were deposited on zirconium (Zr) alloy claddings by using physical vapor deposition (PVD). The Cr-coated samples tested at 1200 o C 1300 C, respectively, for different exposure time in water steam environment. Microstructures compositions of the coating/substrate system after oxidation characterized X-ray diffraction, scanning ele...