The action of a biosensor containing non Michaelis-Menten enzyme was modeled at mixed enzyme kinetics and external diffusion limitation in the case of substrate inhibition. The calculations show that at the electrode surface multi steady-state concentrations of substrate may be generated if diffusion module is much larger than 1 and the substrate bulk concentration is much bigger than Michaelis...

Cinatrins A, B and C3 inhibited phospholipase A2 purified from rat platelets in a dose-dependent manner. Cinatrin C3, the most potent component (IC50 70 microM), was noncompetitive with a Ki value of 36 microM. Cinatrins B and C3 also inhibited both porcine pancreas and Naja naja venom phospholipase A2. Inhibition of rat platelet phospholipase A2 by cinatrin C3 was independent of Ca2+ and subst...

Tension outputs were measured in skinned crayfish muscle fibers exposed to solutions variously buffered for both Mg-adenosine triphosphate (ATP) and Ca. Two types of data are shown, relating tension and substrate concentration with different levels of Ca present, or tension and calcium concentration at different levels of substrate. The data are fitted by curves calculated from a general equati...

Rhizopus oligosporus was used in the present study for the production of protease enzyme under SSF. Sunflower meal was used as by-product of oil industry incorporated with organic salts was employed for the production of protease enzyme. The main purpose of the present was to study different parameters of protease productivity, its yields and to optimize basal fermentation conditions. The optim...

Simultaneous saccharification and fermentation (SSF) of alkaline hydrogen peroxide pretreated Antigonum leptopus (Linn) leaves to ethanol was optimized using cellulase from Trichoderma reesei QM-9414 (Celluclast from Novo) and Saccharomyces cerevisiae NRRL-Y-132 cells. Response surface methodology (RSM) and a three-level four-variable design were employed to evaluate the effects of SSF process ...

Mathematical models which relate the growth rate of a microorganism to a single limiting substrate concentration have long been established. In recent years, it has become apparent that, under certain conditions, the growth rate of an organism may be simultaneously limited by two or more substrates. Mathematical models of double-substrate limitation fall into two categories: interactive and non...

A barbiturate receptor has proven effective in improving selectivity in solid-phase microextraction of barbiturates when doped into plasticized poly(vinyl chloride) (PVC). It would be beneficial to have selective extractions for any given organic species; however, the receptors do not exist. They will be found by screening of libraries of potential receptors; thus, a screening method is needed....