For general Sturm-Liouville operators with separated boundary conditions, we prove the following: If E1,2 ∈ R and if u1,2 solve the differential equation Huj = Ejuj , j = 1, 2 and respectively satisfy the boundary condition on the left/right, then the dimension of the spectral projection P(E1,E2)(H) of H equals the number of zeros of the Wronskian of u1 and u2.