Pyrosequencing of the 16S rRNA targeting RNA, community-level physiological profiles made with Biolog EcoPlates, proteolysis, and volatile component (VOC) analyses were mainly used to characterize the manufacture and ripening of the pasta filata cheese Caciocavallo Pugliese. Plate counts revealed that cheese manufacture affected the microbial ecology. The results agreed with those from culture-...

Antibiotic resistance signifies a safety hazard to public health. Lactic acid bacteria, particularly, Streptococcus thermophilus and Lactobacillus bulgaricus, are useful organisms responsible for dairy fermentation. In keeping with this, they may transfer antibiotic human population. We conducted study exanimated the pattern distribution of genes S. L. bulgaricus strains isolated from tradition...

Çalışmada, 31 Lactobacillus delbrueckii subsp. bulgaricus ve 34 Streptococcus thermophilus bakteri kültürlerinde, o-nitrofenil-beta-D-galaktosit (o-NPG) substrat olarak kullanılarak, β-galaktosidaz enzim spesifik aktiviteleri taranmıştır. L. suşları 0.186-6.500 U/mg arasında aktivite gösterirken, S. 0.172-5.064 göstermiştir. ZN541 (6.500 U/mg) Z1052 (5.064 suşlarının yüksek yeteneğine sahip old...

The polytopic membrane protein FeoB is a ferrous iron transporter in prokaryotes. The protein contains a potassium-activated GTPase domain that is essential in regulating the import of iron and conferring virulence to many disease-causing bacteria. However, the mechanism by which the G-domain of FeoB hydrolyzes GTP is not well understood. In particular, it is not yet known how the pivotal step ...

The 35.5-kb ICESt1 element of Streptococcus thermophilus CNRZ368 is bordered by a 27-bp repeat and integrated into the 3' end of a gene encoding a putative fructose-1,6-biphosphate aldolase. This element encodes site-specific integrase and excisionase enzymes related to those of conjugative transposons Tn5276 and Tn5252. The integrase was found to be involved in a site-specific excision of a ci...

The microbial composition of artisan and industrial animal rennet pastes was studied by using both culture-dependent and -independent approaches. Pyrosequencing targeting the 16S rRNA gene allowed to identify 361 operational taxonomic units (OTUs) to the genus/species level. Among lactic acid bacteria (LAB), Streptococcus thermophilus and some lactobacilli, mainly Lactobacillus crispatus and La...

The Streptococcus thermophilus virulent pac-type phage 2972 was isolated from a yogurt made in France in 1999. It is a representative of several phages that have emerged with the industrial use of the exopolysaccharide-producing S. thermophilus strain RD534. The genome of phage 2972 has 34,704 bp with an overall G+C content of 40.15%, making it the shortest S. thermophilus phage genome analyzed...

Diyet lifleri, tüketici sağlığı üzerine olumlu etkilerinden dolayı kullanımı giderek popülerleşmektedir. Çalışmamızda ayran üretiminde psyllium, bezelye ve yulaf araştırılmıştır. Farklı konsantrasyonlarda diyet lifleri içeren örnekleri +4 °C’de 21 gün boyunca depolanmıştır. Depolamanın 1., 7., 15. 21. günlerinde pH, titrasyon asitliği, kuru madde, kül, yağ, protein, viskozite, tirozin, mineral ...