A new fingerprinting technique with the potential for rapid identification of bacteria was developed by combining proton magnetic resonance spectroscopy ((1)H MRS) with multivariate statistical analysis. This resulted in an objective identification strategy for common clinical isolates belonging to the bacterial species Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, S...

Ten patients received a 30-min infusion of amikacin (30 mg/kg) on day 1 and 15 mg/kg on day 2. Mean serum creatinine was 1.1 +/- 0.3 (standard deviation) mg/dl before and 1.0 +/- 0.3 mg/dl 3 days after the second infusion. Mean serum amikacin concentrations before, at the end of infusion, and 1, 6, 12, and 24 h after 30 and 15 mg/kg were 0, 157, 79, 31, 16, 5, 5, 85, 51, 19, 12, and 5 mg/liter,...

In this report, 30 tetracycline-resistant clinical isolates of group B Streptococcus were examined to assess the extent to which tetracycline resistance is plasmid mediated. Of these, 27 showed no physical or genetic evidence of plasmid-mediated resistance; however, one conjugative and two small (3.5 X 10(6)-dalton) multicopy non-self-transmissible tetracycline resistance plasmids were identifi...

Essential oils from different organs of Ageratum fastigiatum (Gardn.) R. M. King et H. Rob., obtained by hydrodistillation were analyzed through Gas Chromatography and Gas Chromatography/Mass Spectrometry. The essential oils were tested for antimicrobial activity by the agar-diffusion method and minimal inhibitory concentration (MIC). The main compounds found were β-caryophyllene, germacrene D ...

We have designed a universal PCR capable of amplifying a portion of the 16S rRNA gene of eubacteria, including Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus agalactiae, Streptococcus pneumoniae, Enterococcus faecium, Enterococcus faecalis, Mycobacterium tuberculosis, Legionella pneumophila, Escherichia coli, Klebsiella pneumoniae, Serratia marcescens, ...

Patients with chronic fatigue syndrome (CFS) are affected by symptoms of cognitive dysfunction and neurological impairment, the cause of which has yet to be elucidated. However, these symptoms are strikingly similar to those of patients presented with D-lactic acidosis. A significant increase of Gram positive facultative anaerobic faecal microorganisms in 108 CFS patients as compared to 177 con...

propolis has traditionally been used in curing infections and healing wounds and burns. current researches have shown that propolis has antibacterial, antifungal and antiviral actions however, the pharmacological activity of propolis is highly variable depending on its geographic origin. there have been few studies on the effects of iranian propolis on the oral microorganisms. in this in-vitro ...

Fresh flowers of Tagetes erecta L., the flavonoid was isolated by extraction and fractionation with different solvents. It was then identified as patulitrin (10mg/100ml) by various tests. It was then subjected to disc diffusion assay to assess its antibacterial potentials against Alcaligens faecalis, Bacillus cereus, Campylobacter coli, Escherchia coli, Klebsiella pneumoniae, Pseudomonas aerugi...

Three hundred and fourteen strains of group D streptococci were studied by means of a number of tests. The majority of the strains were identified as Streptococcus faecalis (83 strains), Streptococcus faecium (131 strains), or Streptococcus bovis (32 strains). Several strains (47 or nearly 15%) either shared characteristics of two species or were completely atypical. S. faecalis and S. bovis we...

In normal human serum chelated with magnesium (10 mM) and ethylene glycotetraacetic acid (10 mM) (MgEGTA), the classical pathway of complement activation is blocked; however, the alternate pathway of complement activation is intact. Diplococcus pneumoniae, Staphylococcus albus, Streptococcus viridans, Streptococcus faecalis, and Serratia marcescens were opsonized in normal human serum containin...