نتایج جستجو برای: stimulated by his environment

تعداد نتایج: 7350401  

Journal: :Protein expression and purification 2003
Arun K Mohanty Chad R Simmons Michael C Wiener

Affinity tags such as polyhistidine greatly facilitate recombinant protein production. The solubility of integral membrane proteins is maintained by the formation of protein-detergent complexes (PDCs), with detergent present at concentration above its critical micelle concentration (CMC). Removal of the affinity tag necessitates inclusion of an engineered protease cleavage site. A commonly util...

Journal: :Protein expression and purification 1999
C M Hill R G Duggleby

Ketol-acid reductoisomerase (EC 1.1.1.86) catalyzes the conversion of 2-aceto-2-hydroxyacids to 2-keto-3-hydroxyacids and their subsequent reduction by NADPH to 2,3-dihydroxyacids. The gene encoding the Escherichia coli enzyme was cloned and expressed as a hexahistidine-tagged fusion protein and the recombinant enzyme purified by metal-ligand affinity chromatography. The pure enzyme was tested ...

Journal: :The Journal of biological chemistry 2003
Antonio Ferreira-Pereira Sergio Marco Annabelle Decottignies Joseph Nader André Goffeau Jean-Louis Rigaud

Pdr5p, the major multidrug exporter in Saccharomyces cerevisiae, is a member of the ATP-binding cassette (ABC) superfamily. Pdr5p shares similar mechanisms of substrate recognition and transport with the human MDR1-Pgp, despite an inverted topology of transmembrane and ATP-binding domains. The hexahistidine-tagged Pdr5p multidrug transporter was highly overexpressed in yeast strains where other...

Journal: :Health Information Management Journal 2009

Journal: :Microbiology 2003
M M Vickerman M Wang L J Baker

The Streptococcus gordonii glucosyltransferase structural gene, gtfG, is located immediately downstream from its positive transcriptional regulatory determinant, rgg. Recent genetic studies have indicated that the 3' end of rgg is involved either directly as a binding site or indirectly, e.g. by playing a role in secondary structure, in the interaction of Rgg with the gtfG promoter. A previousl...

Journal: :ACS nano 2010
Duane E Prasuhn Juan B Blanco-Canosa Gary J Vora James B Delehanty Kimihiro Susumu Bing C Mei Philip E Dawson Igor L Medintz

One of the principle hurdles to wider incorporation of semiconductor quantum dots (QDs) in biology is the lack of facile linkage chemistries to create different types of functional QD--bioconjugates. A two-step modular strategy for the presentation of biomolecules on CdSe/ZnS core/shell QDs is described here which utilizes a chemoselective, aniline-catalyzed hydrazone coupling chemistry to appe...

2013
Avinash S. Punekar Maria Selmer

Methyltransferase RlmJ uses the cofactor S-adenosylmethionine to methylate the exocyclic nitrogen N6 of nucleotide A2030 in 23S rRNA during ribosome assembly in Escherichia coli. RlmJ with a C-terminal hexahistidine tag was overexpressed in E. coli and purified as a monomer using Ni(2+)-affinity and size-exclusion chromatography. The recombinant RlmJ was crystallized using the sitting-drop vapo...

2013
Mark A. Arbing Sum Chan Liam Harris Emmeline Kuo Tina T. Zhou Christine J. Ahn Lin Nguyen Qixin He Jamie Lu Phuong T. Menchavez Annie Shin Thomas Holton Michael R. Sawaya Duilio Cascio David Eisenberg

The expression of heteroligomeric protein complexes for structural studies often requires a special coexpression strategy. The reason is that the solubility and proper folding of each subunit of the complex requires physical association with other subunits of the complex. The genomes of pathogenic mycobacteria encode many small protein complexes, implicated in bacterial fitness and pathogenicit...

Journal: :Biochemistry 2005
David M Duda Chingkuang Tu S Zoë Fisher Haiqain An Craig Yoshioka Lakshmanan Govindasamy Philip J Laipis Mavis Agbandje-McKenna David N Silverman Robert McKenna

The residue phenylalanine 198 (Phe 198) is a prominent cause of the lower activity of human carbonic anhydrase III (HCA III) compared with HCA II and other isozymes which have leucine at this site. We report the crystal structures of HCA III and the site-directed mutant F198L HCA III, both at 2.1 A resolution, and the enhancement of catalytic activity by exogenous proton donors containing imida...

Journal: :Scientific reports 2016
Veeraraghavan Usha Adrian J Lloyd David I Roper Christopher G Dowson Guennadi Kozlov Kalle Gehring Smita Chauhan Hasan T Imam Claudia A Blindauer Gurdyal S Besra

With the increased incidence of tuberculosis (TB) caused by Mycobacterium tuberculosis there is an urgent need for new and better anti-tubercular drugs. N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE) is a key enzyme in the succinylase pathway for the biosynthesis of meso-diaminopimelic acid (meso-DAP) and L-lysine. DapE is a zinc containing metallohydrolase which hydrolyses N-succinyl ...

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