First, excised human skin obtained from abdominal plastic surgery is prepared for storage. In this procedure, the subcutaneous fatty tissue is separated from the outer skin layers, namely the epidermis and the dermis by a scalpel. Second, the epidermis is separated from the underlying dermis by a heat-separation technique. The separated epidermis will be used for subsequent permeation experimen...

The epidermis of mice treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) was investigated using the method of stereological cytology. These procedures were carried out in order to quantify the morphological changes taking place in basal cells, the presumed target cells of TPA in the epidermis. The basal layer of the treated epidermis was composed of TPA-induced dark basal keratinocytes (DC...

BACKGROUND This study determines the expression of P53 protein and the intensity of immunoreactivity in basal cell carcinoma in comparison with the adjacent nontumoral epidermis in sun-exposed areas of the head and neck regions. The mean age of immunoreactivity in tumoral and adjacent nontumoral epidermis is also determined. METHODS This descriptive-analytical study was performed retrospectiv...

In skin penetration studies, HPLC-MS/MS analysis on extracts of heat-separated epidermis and dermis provides an estimate the amount drug penetrated. this study, MALDI-MSI enabled qualitative distribution endogenous molecules molecule, tofacitinib quantitative in epidermis. The delivery to was investigated a Franz diffusion cell using three different formulations (two oil-in-water creams, C1 C2 ...

To investigate early disease biomarkers for atopic dermatitis (AD) we investigated epidermal cytokine levels and skin microbial profiles. We performed minimally invasive biosampling in a prospective cohort of 50 neonates at high risk developing AD including tape strips swabs from antecubital fossa, volar forearm cheek 3, 6, 12, 18 24 months after birth. 26% the included new-borns developed unti...

Studies on the role of the minerals in epidermal carcinogenesis of mice induced by methylcholanthrene have been described (2), and the need for the determination of the water content of the epidermis became obvious in investigations on the succinic dehydrogenase and cytochrome oxidase activities of the epidermis (3). Since the latter are expressed as cu. mm. of oxygen consumed per mgm. dry weig...