Daptomycin (DAP) is bactericidal against methicillin-resistant Staphylococcus aureus (MRSA) in vitro, but it failed to eradicate MRSA in an experimental model of implant-associated infection. We therefore investigated various factors which could explain treatment failure by evaluating DAP activity, including the role of different cell wall components, adherence, biofilm, and calcium ions (Ca(2+...

Skalka B.: Bacteriocin Activity of Staphylococcus aureus, Staphylococcus intermedius and Coagulase-n.egative Staphylococcal Strains. Acta vet. Brno, 55, 1986: 65-72. A total of 567 staphylococcal strains was examined for bacteriocin activity. It contained 367 S. aureus strains, 114 S. intermedius strains, and 86 coagulasenegative staphylococci belonging to ten species. The deferred method was e...

Staphylococcal colonization of human skin is ubiquitous, with particular species more frequent at different body sites. Whereas Staphylococcus epidermidis can be isolated from the skin of every individual tested, Staphylococcus aureus is isolated from <5% of healthy individuals. The factors that drive staphylococcal speciation and niche selection on skin are incompletely defined. Here we show t...

The ability of Staphylococcus aureus to invade and survive within endothelial cells is believed to contribute to its propensity to cause persistent endovascular infection with endothelial destruction. In the present study, we show that following invasion of human umbilical vein endothelial cells, intracellular S. aureus organisms remain viable over a 72-h period and, as determined by transmissi...

The clinical importance of coagulase-positive staphylococci is well-known. In fact, Staphylococcus aureus is so often considered a pathogen that few laboratories appreciate that up to 25% of these strains isolated from blood cultures are actually contaminants from skin flora.' Conversely, coagulase-negative staphylococci have so long been considered an environmental contaminant that the pathoge...

Milk is considered a nutritious food because it contains several important nutrients including proteins and vitamins. Conversely, it can be a vehicle for several pathogenic bacteria such as Staphylococcus aureus. This study aimed to analyze the frequency of genes encoding the nine Staphylococcal enterotoxins (SEs) and enterotoxin gene profiles in S. aureus isolates derived from raw bovine milk....

In this study, we investigated the interactions of Staphylococcus aureus with mast cells, which are multifunctional sentinels lining the surfaces of the body. We found that bone marrow-derived murine mast cells (BMMC) exerted a powerful phagocytosis-independent antimicrobial activity against S. aureus. Both the release of extracellular traps as well as discharge of antimicrobial compounds were ...

Between January 1997 and December 1999, bloodstream isolates from 15,439 patients infected with Staphylococcus aureus and 6350 patients infected with coagulase-negative Staphylococcus species (CoNS) were referred by SENTRY-participating hospitals in the United States, Canada, Latin America, Europe, and the Western Pacific region. S. aureus was found to be the most prevalent cause of bloodstream...

Nasal colonization with antibiotic resistant bacteria represents both a risk factor for the colonized individual and their immediate contacts. Despite the fact that healthcare workers such as orthopedic surgeons are at a critical interface between the healthcare environment and an at-risk patient population, the prevalence of antibiotic resistant bacteria within the surgical profession remains ...

BACKGROUND/AIM Nosocomial bloodstream infections (BSI) contribute to grater morbidity and mortality rates, as well as to increasing length of hospital stay and health care costs. All patients with nosocomial BSI identified during the one-year period were studed to identify microbiological factors associated with these infections. METHODS A one-year prospective cohort study was performed in pa...