Few have reported a concomitant malignant neoplasm with immunoglobulin G4 (IgG4)-related diseases. We describe a case of lung cancer and gastric cancer accompanied with IgG4-related disease. A 78-year-old man had an area of ground-glass opacity with central collapse in right upper lobe and a gastric cancer. The patient underwent a right upper lobectomy following a gastrectomy for the gastric ca...

The simplest test is the WBC count and differential. White cells can be counted manually in specially designed chambers (Neubauer) or with automated counters. The latter are widely used, offering the advantage of higher accuracy and speed over manual techniques. To determine the differential, a drop of blood is thinly spread over a glass slide, air dried, and stained with a Romanofsky stain, mo...

The bright pigments of cave pictures in Spain have been preserved from Paeleolithic times by the accidental formation of a surface film of calcite (Kuhn, 1952), and since then many glazes have been used deliberately for the protection of colours. The idea of varnishing glass slides is not novel, but practical difficulties concerning preparation, pH, and especially viscosity, have discouraged it...

Noble metal particles have long fascinated scientists because of their intense color, which led to their application in stained glass windows as early as the Middle Ages. The recent resurrection of colloidal and cluster chemistry has brought about the strive for new materials that allow a bottoms-up approach of building improved and new devices with nanoparticles or artificial atoms. In this re...

(usually 2 to 4 specimens depending on their size) excisional specimens from anatomic sites of similar consistency on the same patient may be sectioned together and mounted on one glass slide by the single section method. The single section method saves time by omitting the division each specimen into smaller sections, which reduces the number of specimens that must be cut, stained, and cover s...

INTRODUCTIONThis protocol describes a method for concentrating gel bands or spots excised from 1D or 2D acrylamide gels. Several replicate protein bands (or spots) are often required to provide sufficient material for sequencing. In these situations, it is necessary to excise several stained protein bands or spots from the gels and pre-concentrate them prior to electroblotting for amino-termina...

Whole cells of Actinobacillus pleuropneumoniae (A. pleuropneumoniae) serotype 1, 2, 5 or 7 attached to fibrins were fixed in 10% neutral buffered formalin and embedded in paraffin. The sections on a slide glass were stained by the avidin-biotin complex immunoperoxidase (ABC) method. Test sera were applied to sections as primary antibodies. The serum antibodies against A.pleuropneumoniae (seroty...

Morphology continues to be indispensable for primary isolation and recognition of bacteria. Under controlled conditions of growth, microscopic and gross characteristics of most bacterial species are constant, replicable, and have high differential values. This report is concerned with microculture morphology of Mycobacterium 13 mm) was then placed over each planted area. The plates were incubat...

The midguts and associated muscle fibers of A. quadrimaculatus were dissected out in saline solution and immediately fixed in a solution of 1 % osmium tetroxide in 0.1 M potassium phosphate buffer, pH 7.2, for 5 min at room temperature. The specimens were subsequently dehydrated in ethyl alcohol for 2 hr and embedded in Epon 812 (Luft, 1961). Sections were cut on an LKB ultra microtome with gla...