The core domain of the tumour suppressor p53 is of inherently low thermodynamic stability and also low kinetic stability, which leads to rapid irreversible denaturation. Some oncogenic mutations of p53 act by just making the core domain thermosensitive, and so it is the target of novel anti-cancer drugs that bind to and stabilise the protein. Increasing the stability of the unstable core domain...

Rheological shear forces in the blood trigger von Willebrand factor (VWF) unfolding which exposes the Y1605-M1606 scissile bond within the VWF A2 domain for cleavage by ADAMTS13. The VWF A2 domain contains 2 structural features that provide it with stability: a vicinal disulphide bond and a Ca(2+)-binding site (CBS). We investigated how these 2 structural features interplay to determine stabili...

Resolving molecular determinants of mechanical stability of proteins is crucial in the rational design of advanced biomaterials for use in biomedical and nanotechnological applications. Here we present an interdisciplinary study combining bioinformatics screening, steered molecular dynamics simulations, protein engineering, and single-molecule force spectroscopy that explores the mechanical pro...

To solve the heat equation on parallel computers, a high-order finite difference domain decomposition algorithm is discussed. In this procedure, interface values between subdomains are calculated by the classical explicit scheme, while interior values of sub-domains are determined by the famous forth-order compact scheme. The stability and convergence for this domain decomposition algorithm are...

Considering the linear delay difference system x n 1 ax n Bx n − k , where a ∈ 0, 1 , B is a p × p real matrix, and k is a positive integer, the stability domain of the null solution is completely characterized in terms of the eigenvalues of the matrix B. It is also shown that the stability domain becomes smaller as the delay increases. These results may be successfully applied in the stability...

We report on direct stabilization of a femtosecond laser by a high-finesse passive optical cavity. Detailed comparison of two distinct stabilization schemes leads to new understanding of the optimum conditions for cavity stabilization and limitations on the ability to transfer the frequency stability of the cavity to the microwave domain. The stability of the frequency comb is explored in both ...

There are great demands on the stability, expression yield and resistance to aggregation of antibody fragments. To untangle intrinsic domain effects from domain interactions, we present first a systematic evaluation of the isolated human immunoglobulin variable heavy (V(H)) and light (V(L)) germline family consensus domains and then a systematic series of V(H)-V(L) combinations in the scFv form...