إ ةيرهجملا جماربلا لمشي اذهو ،ةيرورملا ةآرحلا ةاآاحم جمارب روطت يف مهاس ةيتامولعملا ايجولونكتلا يف ريبكلا مدقتلا ن ) Microscopic ( ، نلا ىلع بلطلا ةاآاحم اًضيأ لمشي نايحلأا ضعب يفو ، تاعطاقتو قرط نم اهيف امب لقنلا ةموظنم ةاآاحمب حمسيل يللآا بساحلا تاقيبطت قاطنو لق . ةيرهجملا ةيرورملا ةآرحلا ةاآاحم جمارب نيب نراقتو عجارت ةقرولا هذهو ) Microscopic ( ةيلومشلاو ) Macroscopic ( فلاتخلاا هجوأ ىلع ةزآر...

Background and objectives: DNA hyper-methylation is an important aspect involved in carcinogenesis cancer progression, which affects mainly CpG islands of causes inactivation tumour suppressor genes. Therefore hypermethylation status the genomic both transformed cancerous cell lines carcinogen-induced lung was ascertained by analysis expressions certain major specific The other objective to exa...

AIM To establish a simple and reliable polymerase chain reaction (PCR) methodology for random amplification of whole genomic DNA from limited histopathological samples. METHODS Trace amounts of genomic DNA extracted from fresh tissue and individual lymphoid follicles microdissected from archival paraffin wax tissue sections were amplified using a two-phase PCR protocol with random hexamers as...

Leptospirosis, a disease with protean manifestations, is caused by Leptospira species. Early and reliable identification of Leptospira serovars remains to be a challenge due to diversity at serovar level. The potential of polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) was assessed for detection of sequence variation in pathogenic specific 631 bp fragment of 16S r...

SSCP (single-strand conformational polymorphism) is used widely in the field of human biomedicine, but its potential as a population genetics tool for the recovery of nuclear gene genealogies remains to be realized. We describe and illustrate a use for SSCP in the physical isolation of nuclear haplotypes that circumvents several difficulties associated with more conventional cloning procedures....

complex vertebral malformation(cvm) isanautosomal recessivehereditarydisorder caused bya point mutation in position 559 inexon 4 of the slc35a3 gene on chromosome 3 inholstein dairy cattle.this mutationchanges the function of uridine 5-diphosphate-n-acetylglucosamine transporter protein bythe substitution of valine for phenylalanine at position 180 of this protein. the disease causes premature ...