In this report, we describe a method for the delivery of small interfering RNAs (siRNAs) into plant cells. In vitro synthesized siRNAs that were designed to target the coding region of a GREEN FLUORESCENT PROTEIN (GFP) transgene were applied by various methods onto GFP-expressing transgenic Nicotiana benthamiana plants to trigger RNA silencing. In contrast to mere siRNA applications, including ...

Flame Spray Pyrolysis (FSP) is a well investigated high temperature aerosol technique for synthesizing nano-sized metal oxide catalysts of almost all metals and transition metals by gas-to-particle conversion (Teoh et al. 2010). However, specific designs of multi-component catalysts and the controlled dispersion of catalytic material on the support are limited with this approach. Double Flame S...

Table 1. Effect of paclobutrazol (paclo) on plant height and number of leaves developed on hollyhock at 2, 4, or 6 weeks after treatment. All application techniques were designed to deliver 0.10 mg paclo/10-cm Our objective was to determine the efficacy of a given dosage of β-[(4-chlorophenyl)methyl]-α-(1,1-dimethylethyl)-1H1,2,4-triazole-1-ethanol [paclobutrazol (paclo)] applied using differin...

AIMS To determine haematological parameters, urine mutagenicity (on three Salmonella typhimurium strains), and DNA damage (using the comet assay) in mononuclear leucocytes of farmers before and after a one-day spraying period of pear and apple trees with the fungicide captan in usual conditions. METHODS Fruit growers were exposed to captan during the 1998 (n = 12) and/or the 2000 spraying sea...

We examined whether the spraying of soybean leaves with hydrogen peroxide (H2O2) would alleviate the symptoms of drought stress. Pre-treatment by spraying leaves with H2O2 delayed foliar wilting caused by drought stress compared to leaves sprayed with distilled water (DW). Additionally, the relative water content of drought-stressed leaves pre-treated with H2O2 was higher than that of leaves pr...

In recent years, coating processes based on the impact of high-velocity solid particles such as cold spraying and aerosol deposition have been developed and attracting much industrial attention. A novel coating process called 'warm spraying' has been developed, in which coatings are formed by the high-velocity impact of solid powder particles heated to appropriate temperatures below the melting...

swallow wort is a perennial weed which has caused damage in agricultural fields in iran especially in orchards. therefore, an experiment was conducted during 2009-2011 in order to its integrated management in arachis orchard in abyek based on randomized complete block design with three replications and 18 treatments. the treatments included gramaxon (paraquat 20% sl), roundup (glyphosate 41% sl...

Esteya vermicola (Ophiostomataceae) is an endoparasitic fungus that has great potential as a biological control agent against the pinewood nematode Bursaphelenchus xylophilus which causes pine wilt disease. We tested E. vermicola for control of pine wilt disease by spraying E. vermicola conidia on artificial wounds on pine seedlings, and the optimum E. vermicola treatment density and applicatio...

The effects of 2 concentrations of kinetin, i.e., 2 and 4 mM together with 2 levels of drought stress, i.e., moderate (35% soil saturation capacity) and severe (20% soil saturation capacity) were investigated on the growth parameters of tomato including fresh and dry leaf weight, leaf number, leaf surface area, fruit number, fruit weight, and enzymatic antioxidants. To this end, first tomato pl...

Absorption of the herbicide paraquat (1,1'-dimethyl-4,4'-bipyridinium) by mixer-loaders and spray operators on a Sri Lankan tea plantation was assessed over five consecutive days of spraying. Beginning on the day before spraying started and continuing for each of the five spraying days and for seven days after the last day of spraying, 24-hour urine samples were collected from each of the worke...