In the process of cell division, chromosomes are segregated by mitotic spindles -- bipolar microtubule arrays that have a characteristic fusiform shape. Mitotic spindle function is based on motor-generated forces of hundreds of piconewtons. These forces have to deform the spindle, yet the role of microtubule elastic deformations in the spindle remains unclear. Here we solve equations of elastic...

The GTPase Ran regulates multiple cellular functions throughout the cell cycle, including nucleocytoplasmic transport, nuclear membrane assembly, and spindle assembly. Ran mediates spindle assembly by affecting multiple spindle assembly pathways: microtubule dynamics, microtubule motor activity, and spindle pole assembly. Ran is predicted to facilitate spindle assembly by remaining in the GTP-b...

     In this research, hydrogels based on poly vinyl alcohol and poly acrylic acid blend were prepared which were cross-linked by applied thermal conditions. Afterward, effects of time and heating on water uptake were investigated. The highest water uptake value exhibited by the sample that was heated for 20 min. at 110 ºC was about 2129% after 4 days at equilibrium state. Hydrogels exhibited p...

Spindle cell lipoma is a histologically distinct variant of lipoma characteristically arising in the subcutis of the posterior neck, upper back, or shoulder. Spindle cell lipomas infrequently occur within the oral cavity and, in particular, rarely involve the tongue. The clinical and pathologic features of eight cases of spindle cell lipoma affecting the tongue were analyzed. The study group in...

Ran’s downhill slide during mitosis H asegawa et al. clarify the role of RanGTP in the formation of the mitotic spindle. Because RanGTP is prevalent in the cell, its job during spindle assembly has been difficult to nail down. One possibility is that RanGTP promotes construction of the spindle by liberating spindle assembly factors from the importins that carry them. Previous studies of Xenopus...

Chromosome segregation during mitosis and meiosis relies on the spindle and the functions of numerous microtubule-associated proteins (MAPs). One of the best-studied spindle MAPs is the highly conserved TPX2, which has been reported to have characteristic intracellular dynamics and molecular activities, such as nuclear localisation in interphase, poleward movement in the metaphase spindle, micr...

To maintain tissue architecture, epithelial cells divide in a planar fashion, perpendicular to their main polarity axis. As the centrosome resumes an apical localization in interphase, planar spindle orientation is reset at each cell cycle. We used three-dimensional live imaging of GFP-labeled centrosomes to investigate the dynamics of spindle orientation in chick neuroepithelial cells. The mit...

Compared to epithelial lesions, spindle-cell lesions of the breast are relatively uncommon and, because of that, may cause diagnostic difficulty. The majority of these spindle cell lesions, such as fibromatosis, nodular fasciitis, spindlecell carcinoma, inflammatory myofibroblatic tumor and angiosarcoma, resemble their more common non-mammary counterparts. A few others, such as pseudoangiomatou...

The microtubule spindle apparatus dictates the plane of cell cleavage in animal cells. During development, dividing cells control the position of the spindle to determine the size, location, and fate of daughter cells. Spindle positioning depends on pulling forces that act between the cell periphery and astral microtubules. This involves dynein recruitment to the cell cortex by a heterotrimeric...