TPX2 is a Ran-regulated spindle assembly factor that is required for kinetochore fiber formation and activation of the mitotic kinase Aurora A. TPX2 is enriched near spindle poles and is required near kinetochores, suggesting that it undergoes dynamic relocalization throughout mitosis. Using photoactivation, we measured the movement of PA-GFP-TPX2 in the mitotic spindle. TPX2 moves poleward in ...

The GTPase Ran regulates multiple cellular functions throughout the cell cycle, including nucleocytoplasmic transport, nuclear membrane assembly, and spindle assembly. Ran mediates spindle assembly by affecting multiple spindle assembly pathways: microtubule dynamics, microtubule motor activity, and spindle pole assembly. Ran is predicted to facilitate spindle assembly by remaining in the GTP-b...

The spindle is a highly dynamic molecular machine that mediates precise chromosome segregation during cell division. Spindle size can vary dramatically, not only between species but also between different cells of the same organism. However, the reasons for spindle size variability are largely unknown. Here we show that variations in spindle size can be linked to a precise developmental require...

Xorbiting the spindle he Xorbit microtubule-binding protein, say Hannak and Heald (page 19), keeps the spindle from getting away from its passenger DNA. Xorbit is a spindle-localized microtubule plus-end binding protein whose loss causes defects in spindle formation and chromosome alignment. To understand its specific function, the authors used video microscopy to compare spindle assembly in re...

Primary spindle cell sarcoma in the heart is a very uncommon disease. Although primary atrial or pulmonary vein spindle cell sarcomas have been sporadically reported, pericardial spindle cell sarcoma is rarely seen in currently available data. The commentary here is on a primary pericardial spindle cell sarcoma that was preliminarily misjudged to be left main coronary artery disease.

Chromosome segregation during mitosis is mediated by the mitotic spindle. Formation of this microtubular structure relies on distinct processes such as microtubule nucleation and growth and the consequent focusing of these filaments into spindle poles. Here, we discuss our recent finding that a size-exclusion spindle envelope promotes mitotic fidelity in Drosophila cells in light of distinct sp...

Accurate segregation of genetic material into two daughter cells is essential for organism reproduction, development, and survival. The cell assembles a macromolecular structure called the mitotic spindle, which is composed of dynamic microtubules (MTs) and many associated proteins that assemble the spindle and drive the segregation of the chromosomes. Members of the kinesin superfamily of MT a...

Following learning, increased coupling between spindle oscillations in the medial prefrontal cortex (mPFC) and ripple oscillations in the hippocampus is thought to underlie memory consolidation. However, whether learning-induced increases in ripple-spindle coupling are necessary for successful memory consolidation has not been tested directly. In order to decouple ripple-spindle oscillations, h...

To maintain tissue architecture, epithelial cells divide in a planar fashion, perpendicular to their main polarity axis. As the centrosome resumes an apical localization in interphase, planar spindle orientation is reset at each cell cycle. We used three-dimensional live imaging of GFP-labeled centrosomes to investigate the dynamics of spindle orientation in chick neuroepithelial cells. The mit...