Gellan gum is a widely used commercial material, available in many different forms. Its economic importance has led to studies into the biosynthesis of exopolysaccharide gellan gum, which is industrially prepared in high yields using Sphingomonas elodea ATCC 31461. Glucose-1-phosphate uridylyltransferase mediates the reversible conversion of glucose-1-phosphate and UTP into UDP-glucose and pyro...

Haloalkane dehalogenases are microbial enzymes that cleave a carbon-halogen bond in halogenated compounds. The haloalkane dehalogenase LinB, isolated from Sphingomonas paucimobilis UT26, is a broad-specificity enzyme. Fifty-five halogenated aliphatic and cyclic hydrocarbons were tested for dehalogenation with the LinB enzyme. The compounds for testing were systematically selected using a statis...

Aromatic compounds with alkyl substituents are abundant in fossil fuels. These compounds become important environmental sources of soluble toxic products, developmental inhibitors, etc. principally through biological activities. To assess the effect of methyl substitution on the completeness of mineralization and accumulation of pathway products, an isolate from a phenanthrene enrichment cultur...

To the Editor: The genus Sphingomonas was proposed by Yabuuchi et al. in 1990 (1) and amended by Takeuchi et al. in 1993 (2). It now has been subdivided into 4 separate genera: Sphingomonas sensu stricto, Sphingobium, Novosphingobium, and Sphingopyxis. The bacteria of the genus Sphingomonas are yellow-pigmented, nonfermenting, gramnegative bacilli with a single polar fl agellum; they are widely...

The inhibition of the model enzyme, haloalkane dehalogenase from Sphingomonas paucimobilis, was investigated by a combination of electrophoretically mediated microanalysis with a partial filling technique, followed by indirect or direct detection. In this setup, part of the capillary is filled with a buffer suitable for the enzymatic reaction (20 mM glycine buffer, pH 8.6) whereas the rest of t...

Abstract Antibiotic resistance of pathogens is a serious issue that affects health. The airborne particle molecules such as (PM 2.5 , PM 10 ) spread by dust storms can increase the antibiotic in bacterial community. In this study air samples were collected and air-borne bacteria cultured on specific growth media portable sampler device (SAS). Sixty plates used through atmospheric which included...