Abstract Background Nano-fibrous scaffolds provide a three-dimensional matrix that guides sufficient orientation of seeded cells similar to natural niche. In this research, we designed silk scaffold improve the differention mouse spermatogonial stem spermatogenic cell lines. Spermatogonial were collected from neonatal (2–6 days) testes ( n =60) using two steps mechanical and enzymatic method. C...

Children are vulnerable to environmental mutagens, and the developing germline could also be affected. However, little is known about whether exposure to environmental mutagens in childhood will result in increased germline mutations in subsequent adult life. In the present study, male transgenic lacI mice at different ages (7, 25 and 60 days old) were treated with a known environmental mutagen...

OBJECTIVE To determine the diagnostic features of Robertsonian (Rob) translocation (11; 13) in mice and the mechanisms underlying the effect on spermatogenesis and reproductive decline. METHODS A Rob translocation (11; 13) mouse model was established by cross-breeding, and confirmed by chromosome analysis. Chromosome aberrations and translocation patterns were identified in mice with Rob tran...

Introduction: Dravya sangrahana is one of the criteria where different seasons are indicated for collecting parts plants. As per ancient drug collection practice roots to be collected in Hemanth-Shishira (winter) or Greeshma rutu(summer). Eranda(Ricinus communis) an important medicinal plant which particularly as Vrishya (spermatogenic). Hence study has been planned conduct a comparative sperma...

PURPOSE Anti cancer drugs is one of the most important chemotherapeutic factors which can influence spermatogenesis process and germinal epithelium. Since dividing cells are mainly affected by anticancer drugs, the aim of the present study is to investigate the preventive effect of GnRH antagonist on spermatogenic defect produced by anticancer drugs. METHODS In the present study thirty adult ...

Quantitative histology of the descended testis of unilateral cryptorchid bucks was compared with testis of normal bucks to evaluate the reproductive potentials of the scrotal testis in unilateral cryptorchids, using light microscopy techniques. The contralateral scrotal testes of the unilateral cryptorchids and the testes of the normal bucks contained profiles of seminiferous epithelium and eac...

STUDY QUESTION What are the reference values for delineating altered somatic cell gene expression from transcript enrichment/dilution in gene expression studies of human spermatogenesis? SUMMARY ANSWER We have designed a crosstable and rule-of-thumb values for different stages of spermatogenic impairment that define the reference cut-off values for altered gene expression in Sertoli and Leydi...