The aim of the study was to standardize the pellet cryopreservation procedure for chicken semen. Mericanel della Brianza male chicken breeders (Italian breed) were used. Pooled semen samples were processed according to the following conditions: (1) dilution in prefreezing extender to 1 versus 1.5 bill cells/mL sperm working concentration (SWC); (2) 6% versus 9% dimethyl acetamide (DMA) concentr...

BACKGROUND Control of the medium osmolarity and temperature during long or short time sperm manipulation is essential. OBJECTIVE The objectives of the present study were to find the effects of different osmolarities of modified Tyrode's solution and milk on the bull sperm during incubation at above zero temperatures. MATERIALS AND METHODS Semen samples were collected twice from five Najdi b...

background: industrial copper ingest is a common form of poisoning in animals. zinc has an important role in the physiology of spermatozoa, in sperm production and viability. objective: this study was set to investigate whether the adverse effects of long term copper consumption on quality of rat spermatozoa could be prevented by zinc therapy. materials and methods: forty eight mature (6-8 week...

Preservation of canine spermatozoa at 50C is a helpful method for dog breeding management. However, in many species, oxidative damage due to generation of reactive oxygen species (ROS) and long term storage which specifically cause DNA damage, and lipid peroxidation during preservation techniques can cause a decline of sperm motility and fertility capacity. In this study, the effect of antioxid...

The genotoxicity of the ethanolic extract betel nut was evaluated using sarcoma 180 tumour bearing mouse considering sperm motility, viability, biochemical estimation fructose in seminal fluid and head morphology assays. Sperm studied by H-E staining Toluidine blue method. But method is a reliable to evaluate DNA damage sperms. Ethanolic BNE (betel extract) can suppress percentage viability lev...

introduction: the purpose of this research was to study the effects of two cryoprotectants on viability, motility, morphology and fertilization rate of mouse sperm after freezing-thawing. material and methods: spermatozoa were isolated from caudae epididymis of nmri male mice (8 to 10 weeks-old) and divided to three groups of control, experimental 1 and 2. cryoprotectant solution for the first ...

BACKGROUND The predominant phytoestrogen in soy and derived products is the isoflavone Genistein. Genistein has antioxidant properties. Morphine is a main psychoactive chemical in opium that can increase the generation of free radicals and therefore it could adversely affects the spermatogenesis. OBJECTIVE The main goal was to investigate whether the Genistein could protect morphine adverse e...

The aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (Bubalus bubalis) bulls. Five healthy buffalo bulls (5 ejaculates from each bull) were used. Each ejaculate was diluted at 37 ˚C with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 μg mL-1 sodium selenite and the sperm motility and...

Cyclophosphamide (CP) is one of the anti-neoplastic drugs. Despite its numerous clinical applications, it has devastating effects on the testicles and declines the sperm quality in treated patients. This study was aimed to investigate the protective effect of crocin in improving the toxicity induced by CP in reproductive system. In this study, 24 male adult mice (6 to 8 weeks) were randomly div...