introduction: cd59 is a complement control protein (ccp) wich exhibits its inhibitory functions by prevention of complement attack complex (max) formation. the aim of this normozoospermia, olig-astheno and azoospermia. materials and methods: the semen of 57 individual were grouped to normozoospermia (8), asthenozoospermia (21), oligo-astenozoospermia (16), and azoospermia (12) according to who ...

Serum is used as an additive in the preparation of human spermatozoa for fertilization in vitro, as it is superior to other body fluids in supporting sperm motility. We recently purified the major sperm activating macromolecule present in serum and showed it to be a complex of immunoglobulin and apolipoprotein A-I. This complex, which we named sperm activating protein (SPAP), has now been furth...

Toona sinensis leaf (TSL) is commonly used as a vegetable and in spice in Asia. In this study, feeding with aqueous extract of TSL (TSL-A) alleviated oxidative stress and recovered the motility and functions of sperm in rats under oxidative stress. Protein expressions in testes identified by proteomic analysis and verified by Western blot demonstrated that TSL-A not only downregulated the level...

Past studies have suggested that mouse sperm surface galactosyltransferase may participate during fertilization by binding N-acetylglucosamine (GlcNAc) residues in the zona pellucida. In this paper, we examined further the role of sperm surface galactosyltransferase in mouse fertilization. Two reagents that specifically perturb sperm surface galactosyltransferase activity both inhibit sperm-zon...

We analyzed the gene expression of sperm flagellum-movement associated proteins, namely, adenylate kinase domain containing protein (RGD1303144) and outer dense fiber protein 1. These gene expressions were up-regulated in a maturing rat testis, and RGD1303144 gene was expressed dominantly in spermatocytes. Although 2,3,7,8-tetrachlorodibenzo-p-dioxin administration reduced sperm motility, these...

GRP78, a resident endoplasmic reticulum (ER) chaperone involved in protein transport, folding and assembly, has been reported in sperm. It is shown to be localized in the neck region of human sperm. We have previously reported GRP78 to be less phosphorylated in asthenozoosperm.The present study aimed to determine whether sperm GRP78 undergoes phosphorylation changes during epididymal maturation...

Background: Application of follicular fluid (FF) and platelet-activating factor (PAF) in artificial insemination improves sperm motility. Lactate dehydrogenase C (LDH-C) is a key enzyme for sperm motility. In this study, the effects of FF and PAF on the sperm motility index and LDH-C expression were investigated. Moreover, LDH-C expression was compared between asthenozoospermic and normozoosper...

Background: Infertility is an emotional crisis with physical economic and psychosocial challenge because it interferes with all personal life aspects. Infertility can be due to many reasons Many hypotheses have been proposed to explain this including issues of genetics, physiology, nutrition and management, Consuming vegetable protein (soy) instead of carbohydrates or animal protein is associat...

Background: Human β-defensin 126 is a small cationic glycoprotein that coats the plasma membrane of sperm during epididymal transit. It provides protection for sperms from infection-causing microbes and against the female immune system. Defensin remains on the sperm until sperm become capacitated in the female reproductive tract. DEFB126 gene is located on the subtelomeric end of 20p13 in human...