Soluble N-ethylmalameide-sensitive factor attachment protein receptor (SNARE) proteins are present in mammalian sperm and could be involved in critical membrane fusion events during fertilization, namely the acrosome reaction. Vesicle-associated membrane protein/synaptobrevin, a SNARE on the membrane of a vesicular carrier, and syntaxin 1, a SNARE on the target membrane, as well as the calcium ...

In pigs, the damaged sperm membrane leads to leakage of phospholipase C-ζ (PLCζ), which has been identified as a sperm factor, and a reduction of oocyte-activating ability. In this study, we investigated whether sperm selected by Percoll gradient centrifugation (Percoll) have sufficient PLCζ, and whether the efficiency of fertilization and blastocyst formation after intracytoplasmic sperm injec...

BACKGROUND Erectile dysfunction, ejaculatory dysfunction and poor semen quality are the main causes of infertility in men with spinal cord injury (SCI). Different sperm retrieval techniques such as penile vibratory stimulation (PVS), electro-ejaculation (EEJ) or surgical sperm retrieval (SSR) associated or not with sperm cryopreservation can be offered to these patients to preserve their fertil...

In contrast to mice, in sheep no genome-wide demethylation of the paternal genome occurs within the first postfertilization cell cycle. This difference could be due either to an absence of a sheep demethylase activity that is present in mouse ooplasm or to an increased protection of methylated cytosine residues in sheep sperm. Here, we use interspecies intracytoplasmic sperm injection to demons...

The incorporation of radioactivity into various cells in the sequence of spermatogenesis was measured by preparing highly purified spermatozoan nuclei from the cauda epididymidis of mice at daily intervals after injection of (3H)thymidine. The stages of differentiation of these sperm at the time of thymidine administration were calculated from the kinetics of spermatogenesis. The procedure for ...

objective: selection of sperm for intra-cytoplasmic sperm injection (icsi) is usually considered as the ultimate technique to alleviate male-factor infertility. in routine icsi, selection is based on morphology and viability which does not necessarily preclude the chance injection of dna-damaged or apoptotic sperm into the oocyte. sperm with high negative surface electrical charge, named “zeta ...

ABSTRACT In mammals, sperm-borne regulators can be transferred to oocytes during fertilization and have different effects on the formation of pronuclei, first cleavage zygotes, development preimplantation embryos even metabolism individuals after birth. The regulatory role sperm microRNAs (miRNAs) in bovine has not been reported detail. By constructing screening miRNA expression libraries, we f...

Globozoospermia syndrome is a rare teratozoospermia with an incidence below 0.1%. Presence of 100% round sperm heads, absence of acrosome, and messy sperm body and tail are the main characteristics of this syndrome. The activation ability of oocytes and consequently the fertilization rate decrease due to the absence of acrosome. The pathogenesis of this syndrome is not clear [1-2]. Globozoosper...

OBJECTIVES Increased levels of nitric oxide (NO) in the testicular veins of people suffering from varicocele have already been reported. However, the role of NO-synthase (NOS) isozymes and their inhibitors have not been extensively studied. We aimed to evaluate the inhibitory effects of aminoguanidine (AG), on sperm motility, vitality, and mitochondrial membrane potential (MMP) in varicocelized...

OBJECTIVE To evaluate the effect of artificial oocyte activation (AOA) on intracytoplasmic sperm injection (ICSI) cycles using surgically retrieved sperm. DESIGN Laboratory study. SETTING Fertility/assisted fertilization center. PATIENT(S) Couples undergoing surgical sperm retrieval for ICSI (n = 204). INTERVENTION(S) Application of calcium ionophore A23187 for AOA. MAIN OUTCOME MEASU...