Present study was designed to assess the effect of catalase on post-thaw semen quality parameters (SQPs), sperm kinematics, antioxidant and oxidative stress profiles cholesterol efflux in mithun (Bos frontalis). A total 25 ejaculates were selected based biophysical each sample split into four equal aliquots diluted (to get final concentration 60 million spermatozoa per mL) with TCG extender con...

Centrifugal countercurrent distribution (CCCD) in an aqueous two-phase system (TPS) is a resolute technique revealing sperm heterogeneity and for the estimation of the fertilizing potential of a given semen sample. However, separated sperm subpopulations have never been tested for their fertilizing ability yet. Here, we have compared sperm quality parameters and the fertilizing ability of sperm...

BACKGROUND We hypothesized that infertile men with varicoceles have molecular/genetic defects that interact with varicoceles to induce infertility. Studies directly on testis tissue appeared to be a way to link histology, markers for molecular/genetic defects and spermatogenesis, but testis biopsies may induce morbidity. In this report, we present safety and efficacy data on ultrasonically guid...

Abstract Study question Is SIRT1 present in sperm and thus involved fertilization and/or epigenetic code? Do the SIRT1-driven marks be transmitted to progeny? Summary answer localized at head influences code via its deacetylation activity. SIRT1-modulated epigenome is zygotes blastocysts. What known already The addition or removal of acetyl group from lysine residua (i.e. acetylation) post-tran...

Exercise improves health but few data are available regarding benefits of exercise in offspring exposed to developmental programming. There is currently a worldwide epidemic of obesity. Obesity in pregnant women predisposes offspring to obesity. Maternal obesity has well documented effects on offspring reproduction. Few studies address ability of offspring exercise to reduce adverse outcomes. W...

In order to study the genetic control of semen quality parameters, we derived a set of recombinant inbred (RI) mice from crosses between two inbred strains, KE and CBA/Kw, which differ significantly in gamete quality and fertility parameters. In this work, we used male mice from the two parental strains and from ten RI strains to map genes controlling quantitative traits such as sperm mitochond...

PLoS Biology | http://biology.plosjournals.org Epigenomics is one of the many ‘omics’ that is being talked about in the wake of the Human Genome Project. But what is an epigenome, and why have the Wellcome Trust Sanger Institute (Hinxton, United Kingdom) and Epigenomics AG (Berlin, Germany) recently announced the launch of the Human Epigenome Project (HEP), a five-year undertaking during which ...

Abstract Background Renal cell carcinoma (RCC) is the fourteenth most common cancer worldwide, accounting for approximately 4% of all cancers. More than 70% RCC are clear (ccRCC). To date, no reliable biomarkers detection ccRCC have been identified. The aim this study was to identify blood-based DNA methylation (DNAm) markers early and treatment ccRCC. Results ccRCC-associated DNAm markers, we ...