Semen analysis (SA) is the cornerstone for male infertility evaluation. Variations in sperm quantity and quality may make SA an unreliable decision-making tool in providing an insight of infertility (1). Consequently, efforts have been made to upgrade techniques of male fertility analysis. In recent times, primary investigative techniques have centered around assessing sperm capacity, sperm fun...

objectives: the aim of the present study was to evaluate protective effect of royal jelly on sperm parameters, testosterone level, and malondialdehyde (mda) production in mice. materials and methods: thirty-two adult male nmri mice weighing 30±2 g were used. all the animals were divided into 4 groups. control group: received saline 0.1 ml/mouse/day orally for 30 days. royal jelly group (rj): re...

Male infertility can be treated by surgical procedures (e.g., varicocelectomy) or by administration of drugs if causal factors (e.g., seminal tract infections) are detected. In more severe cases, methods of assisted fertilization often have to be applied, but even these have only a limited success rate. Recent studies have demonstrated that disturbances of sperm DNA integrity (determined by the...

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. The number of articles on the clinical significance of sperm DNA integrity and/or fragmentation has been increasing during the last 2 decades. Despite substantial evidence, official recommendation to introduce sperm genomic integrity tests into the routine evaluation of ‘male factor’ is lacking. The review by Agarwal ...

Infertility is considered as one of the major problems associated with spinal cord injury (SCI). However, the exact underlying mechanism is still unknown. Therefore, the main objective of this experimental study was to evaluate the effect of chronic SCI on sperm parameters as well as chromatin integrity and DNA of spermatozoa aspirated from cauda epididymis of rats. Forty-five adult Wistar rats...

Deoxyribonucleic acid was influenced by sperm ageing and sperm storage temperature. In this study, the effect on DNA integrity at pre-freeze and post-freeze stages was analyzed by single cell gel electrophoresis during winter and summer seasons. Traditionally, the diagnosis of male infertility depends on microscopic assessment and biochemical assays to evaluate quality of semen. These tests are...

DNA integrity in sperm is essential for the accurate transmission of genetic information and therefore the maintenance of good health in future generations. The ELISA and Comet assays, two techniques that detect DNA damage in cells, are compared in this study of DNA integrity in human sperm. Both techniques rely on alkaline unwinding for the release of single strands of DNA from the nucleus. Th...

background: evaluating the significance and the effects of plant-derived drugs on laboratory animal’s fertility was recognized. there was antioxidant activity reported from heracleum persicum (golpar).objective: current study aims to study the antioxidant effect of golpar extracts on sperm parameters and chromatin quality in mice.materials and methods: eighteen adult male mice were divided to 3...

(Abstracted from Hum Reprod 2022;37:1106–1125) With intracytoplasmic sperm injection (ICSI), many natural barriers that would normally prevent the entry of abnormal are lost, increasing importance DNA integrity for successful in vitro fertilization. There is currently no consensus on optimal assay measuring quality (DNAq), defined as any structural aspect chromatin can compromise function if di...

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