Spectrophotometric methods were developed according to Quality by Design (QbD) approach as per ICH Q8(R2) guidelines for estimation of dolutegravir. QbD was carried out varying various parameters, and these variable parameters designed into Ishikawa diagram. The present work deals with the development sensitive, simple, accurate, precise cost-effective UV-spectrophotometric method determination...

Cholinesterase (ChE) enzymes have been identified as diagnostic markers for Alzheimer disease (AD). Substrate-based probes synthesised to detect ChEs but they not detected changes in ChE distribution associated with AD pathology. Probes are typically screened using spectrophotometric methods pure enzyme specificity and kinetics. However, the biochemical properties of pathology altered. The pres...

Endogenous shikimic acid determinations are routinely used to assess the efficacy of glyphosate in plants. Numerous analytical methods exist in the public domain for the detection of shikimic acid, yet the most commonly cited comprise spectrophotometric and high-pressure liquid chromatography (HPLC) methods. This paper compares an HPLC and two spectrophotometric methods (Spec 1 and Spec 2) and ...

  Abstract: Spectrophotometric studies were successfully used in quantitative analysis of Amitriptyline Hydrochloride (ATH) Two new methods using spectrophotometry are described for the determination of (ATH) with potassium bromate as the oxidizing agent and acid dyes, Methyl orange and Indigo carmine. Both spectrophotometric methods are based o...

The classical work of Keilin and Hartree (1)) based primarily on microspectroscopic and enzymatic studies, has served as a foundation for our present understanding of terminal electron transport. The application of spectrophotometric techniques was generally limited to studies of soluble purified pigments such as cytochrome c, or of preparations treated with clarifying agents such as sodium cho...

Two simple, rapid, accurate, precise, reliable and economical spectrophotometric methods have been proposed for determination of two widely used new steroids in bulk as well as in pressurised metered dose preparations. The steroids are Ciclesonide and Fluticasone propionate. Both steroids are widely used in the treatment of various types of respiratory disorders. The developed methods for deter...

During the investigation of aviation accidents, postmortem samples from accident victims are submitted to the FAA's Civil Aerospace Medical Institute for toxicological analysis. In order to determine if an accident victim was exposed to an in-flight/postcrash fire or faulty heating/exhaust system, the analysis of carbon monoxide (CO) is conducted. Although our laboratory predominantly uses a sp...

Two simple, rapid and sensitive spectrophotometric methods for the determination of three fluoroquinolones, namely levofloxacin, norfloxacin and ciprofloxacin have been performed either in pure form or in their tablets. In the first method, levofloxacin and norfloxacin are directly treated with bromocresol green (BCG) in dichloromethane while ciprofloxacin is allowed to react with the same dye ...

Seven drugs viz., Paroxetine, Famotidine, Quetiapine, Dextromethorphan, Levofloaxacin, Irbesartan and Itraconazole were tested for the formation of charge transfer complexes with DDQ and the interaction formed a basis for spectrophotometric determination of the drugs. Acetonitrile was found to be suitable solvent for the analysis. The methods have been validated in terms of ICH guidelines The c...

A simple, reliable and rapid method for the spectrophotometric determina­ tion of carboxyhemoglobin in fresh blood is described. A blood hemolysate is prepared by diluting a blood sample with 0.4 percent NH4 OH. Addition of sodium dithionite deoxygenates hemoglobin (and reduces methemoglobin if present) without affecting carboxyhemoglobin. Comparison of the 541/555 nm absorbance ratio for the h...