Oxidation of thin multilayered films of undecylenic (10-undecenoic) acid by gaseous ozone was investigated using a combination of spectroscopic and mass spectrometric techniques. The UV absorption spectrum of the oxidized undecylenic acid film is significantly red-shifted compared to that of the initial film. Photolysis of the oxidized film in the tropospheric actinic region (lambda > 295 nm) r...

The development of rapid, sensitive, and accurate mass spectrometric methods formeasuring peptides, proteins, and even intact protein assemblies hasmade mass spectrometry (MS) an extraordinarily enabling tool for structural biology. Here, we provide a personal perspective of the increasingly useful role that mass spectrometric techniques are exerting during the elucidation of higher order prote...

A new database search algorithm has been developed for identification of intact cross-links in proteins and peptides from tandem mass spectrometric data. Using this algorithm, intact cross-links can be identified and characterized in proteins and peptides with high confidence. The algorithm was tested using BS(3) (bis[sulfosuccinimidyl] suberate) cross-linked Cytochrome C. Five cross-links were...

A precise and rapid method for identifying sites of interaction between proteins was demonstrated; the basis of the method is direct mass spectrometric readout from the complex to determine the specific components of the proteins that interact--a method termed affinity-directed mass spectrometry. The strategy was used to define the region of interaction of a protein growth factor with a monoclo...

In this work, a microfluidic chip-based valveless flow injection analysis (FIA) system with gravity-driven flows and liquid-core waveguide (LCW) spectrometric detection was developed. Automated sample injection in the 0.3-6.4 nL range under gated injection mode was achieved by controlling the vertical position of the waste reservoir fixed on a moving platform and the residence time of the reser...

The cavity ringdown technique has been implemented for electronic spectroscopy of jet-cooled CuSi produced in a pulsed UV laser vaporization plasma reactor. A time-of-flight mass spectrometer is used to simultaneously monitor species produced in the supersonic expansion and allows correlation studies to be performed. Seven rotationally resolved vibronic bands have been measured near 400 nm, yie...

The application of mass spectrometric techniques for the analysis of the complex mix‐ tures inherent in fuel and lubricant samples will be examined. These samples because they are naturally complex mixtures, typically requires either very high resolution mass spectrometry or one of the hyphenated techniques gas chromatography‐mass spectrometry(GC‐MS) or liquid chromatography‐mass spectrometry (...

Unknown compounds in environmental samples are difficult to identify using standard mass spectrometric methods. Fourier transform mass spectrometry (FTMS) has revolutionized how environmental analyses are performed. With its unsurpassed mass accuracy, high resolution and sensitivity, researchers now have a tool for difficult and complex environmental analyses. Two features of FTMS are responsib...

INTRODUCTION Spectroscopic and spectrometric analysis of biological samples is regarded as quick, cost effective, easy to operate, and spectroscopic sample preparation involves minimal sample preparation. RESULTS Techniques like infrared (IR) spectroscopy, surface-enhanced laser desorption/ionization (SELDI)-mass spectroscopy (MS), and matrix-assisted laser desorption/ionization (MALDI) -MS c...

The amino acid sequence of the antitumor protein neocarzinostatin was revised on the basis of mass spectrometric studies. Gas chromatographic mass spectrometry on the O-trimethylsilyl polyaminoalcohol derivatives of peptide mixtures derived from tetra S-carboxymethyl-neocarzinostatin were used to partially sequence neocarzinostatin. In addition, fast atom bombardment-mass spectrometric experime...