We have applied a color-developing reagent, p-nitrobenzene diazonium fluoroborate (diazo reagent) as a post-column derivatization tool for the specific determination of acetoacetate (AcAc) in high performance liquid chromatography (HPLC). A mobile phase consisting of 50 mM KH(2)PO(4), 4 mM tetra-n-butylammonium phosphate (TBAP) as an ion-pair reagent and 2 v/v% methanol, pH 3.5, diazo reagent s...

The determination of serum albumin with the dye reagent bromocresol purple was investigated. We found that bromocresol purple is not a specific reagent for albumin, but that serum proteins in the alpha-, beta-, and gamma-globulin fractions also react with this dye. Similar results were obtained for bromocresol green. Furthermore there is a great difference in absorbance between human and bovine...

Human prostate specific antigen (PSA) standard solution and prostate specific antibody (Ab1 and Ab2) were purchased from Shenggong Bioengineering Ltd Company (Shanghai, China). Glutaraldehyde (25% aqueous solution), bovine serum albumin (BSA), (3-aminopropyl) triethoxysilane (APTES), Tri(2,2′-bipyridyl) dichlororuthenium (II) hexahydrate, and chitosan (CS) were obtained from Sigma-Aldrich Chem....

This paper reports a procedure for the specific radiolabeling of the amino termini of proteins. By Edman degradation, a protein is protected at all lysine amino groups while retaining a free amino terminus and such a modified protein is end-labeled by an amino group-specific reagent (radioiodinated Bolton-Hunter reagent). Partial proteolyses with a variety of specific amino acid cleaving reagen...

Veterinary diagnostic test kits are used in clinical practice and production medicine as a basis for prescribing vaccination and/or treatment regimens. These kits are also used by state and federal animal health officials to control or regulate the movement of animals. The regulation of such kits, which generally takes the form of establishing requirements for licensure, ensures that the kits c...