HIGHLIGHTS Chimeric protein P48-40 of Mycoplasma agalactiae was designed and expressed in E.coli BL21 (DE3). highly soluble form purified with yield 33mg/Lby using Nickel resin. Novel showed high performance for detection specific antibodies 100% sensitivity specificity.

Objective(s): Production of a recombinant and immunogenic antigen using dengue virus type-3 envelope protein is a key point in dengue vaccine development and diagnostic researches. The goals of this study were providing a recombinant protein from dengue virus type-3 envelope protein and evaluation of its immunogenicity in mice. Materials and Methods: Multiple amino acid sequences of different i...

this study was conducted to determine the chemical composition and metabolizable energy of poultry by-product meal (pbpm) from two slaughterhouses in iran. samples were analyzed for dry matter, crude protein, ash, ether extract and gross energy. the amounts of calcium, phosphorus, sodium, potassium, magnesium, iron, manganese, copper and zinc were determined. the apparent metabolizable energy (...

Ageratum houstonianum occurs as a common agricultural weed, it is managed in small and large cropping situation by tillage can contribute to soil fertility waste mulch. The aim of this study was employ the pharmacognostic taxonomic parameters evaluate leaves houstonianum. were collected, identified, air dried, pulverized subjected microscopy, micromeritics, chemomicroscopy, fluorescence, solubl...

Since 1911, when Osborne and Mendel published t,heir first work on “Feeding experiments with isolated food substances,” there has been made accessible to investigation the problem of the biological evaluat’ion of proteins as correlated with their chemical analyses. It is true that for a time t.he advance made in the solution of this problem was obscured by the lack of appreciation of the necess...

Various kinds of fermented soybean meal are now commercially available, whereas the quality these products is uneven due to different processing technologies, fermentation strains, and raw meal, which would lead effects on bioavailability nutrients in aquaculture feeds. Thus, a 10-week feeding trial was conducted evaluate six available (FSBM) growth performance juvenile turbot (Scophthalmus max...