Background: Dense granule antigens (GRA antigens) of Toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. Previous studies reported expression of amino terminal GRA8 protein in fusion with large tags such as glutathione-S-transferase. The present study aimed to produce soluble full length immunogenic GRA8 in bacteria. Methods: GRA8 comple...

β2m: β2-Microglobulin; β2m-free HC: β2m-free Heavy Chain; CTL: CD8+ Cytotoxic T Lymphocytes; DSA: Donor Specific Antibodies; HC: Heavy Chain Polypeptide; HLA: Human Leukocyte Antigens; HLA-I: Human Leukocyte Antigen Class I; HLA-II: Human Leukocyte Antigen Class II; IFNγ: Interferon-γ; kDa: kilodalton; MHC: Major Histocompatibility Complex; MMP: Matrix Metalloproteinase; rHLA: Recombinant HLA; ...

Antigenic compounds from the surface of leukemic myeloblasts are shed in vitro on short-term culture. Blast cells radiolabeled by lactoperoxidase iodination release soluble compounds that react immunologically with alloantisera to leukemia-associated antigens. Partially characterized soluble antigens were used to raise heteroantisera in monkeys that are selectively reactive with leukemic myelob...

Topical antigen application is a focus of current vaccine research. This immunization route mimics natural antigen exposure across a barrier tissue and generates T cells imprinted for skin-selective homing. Soluble antigens introduced through this route require cross-presentation by DC to generate CD8 T cell responses. Here we have explored the relative contribution of various skin-derived DC s...

The specific detection of the antigens of vaccine virus in cultured human cells by means of fluorescent antibody is reported. The antigenic material occurred in the cytoplasm in progressively increasing amounts and in the early stages was in close contact with the nuclear membrane. The antigens were present in a finely particulate form, either as foci or loosely spread out, up to late stages of...

Some patients with Schistosoma mansoni and S. haematobium infections were shown to have soluble schistosomal antigen in their plasma. Antibody to this antigen and to other antigens of adult worms were also present. These findings may be relevant to schistosomal-related immune-complex disease, especially the nephrotic syndrome.

Little has been reported on the serological relationship of halophilic bdellovibrios (Bd). Immunodiffusion analysis performed with rabbit or mouse Bd antisera developed against eight halophilic Bd isolates and one terrestrial Bd isolate, when reacted with soluble antigen preparations of 45 isolates of halophilic Bd, allowed separation into seven serogroups, which were distinct from the terrestr...

The priming of an immune response against a major histocompatibility complex class I-restricted antigen expressed by nonhematopoietic cells involves the transfer of that antigen to a host bone marrow-derived antigen presenting cell (APC) for presentation to CD8+ T lymphocytes. Dendritic cells (DC), as bone marrow-derived APC, are first candidates for presentation of tumor-associated antigens (T...

A heterogenetic antibody showing fixation of complement with human liver and agglutination of sheep erythrocytes was found in certain cases of acute infective hepatitis. The antigen concerned in these reactions was apparently heat stable and alcohol soluble. Differences from other heterogenetic antigen-antibody systems have been noted. The possible relation of the heterogenetic antibody to live...

The priming of an immune response against a major histocompatibility complex class I-restricted antigen expressed by nonhematopoietic cells involves the transfer o f that antigen to a host bone marrow-derived antigen presenting cell (APC) for presentation to CD8 + T lymphocytes. Dendritic cells (DC), as bone marrow-derived APC, are first candidates for presentation o f tumorassociated antigens ...