This study was aimed at evaluating the effect of crop rotation and various tillage systems onthe chemical and biological properties of soil in the years 2013-2015. The first order factorincluded cropping systems: a) crop rotation (pea – winter wheat – spring wheat) and b)monoculture of winter wheat, whereas the second order factor were tillage systems:1) conventional (CT), 2) reduced (RT) and 3...

The nickel-containing enzyme urease is an essential colonization factor of the gastric pathogen Helicobacter pylori, as it allows the bacterium to survive the acidic conditions in the gastric mucosa. Although urease can represents up to 10% of the total protein content of H. pylori, expression of urease genes is thought to be constitutive. Here it is demonstrated that H. pylori regulates the ex...

Importance of urease activity on pathogenic differences among Brucella species was evaluated. In cell-free extracts, the B. suis urease showed 12 times greater specific activity than the B. melitensis urease. When Fisher-344 rats were inoculated intraperitoneally (IP), at 1 week post-inoculation (PI), B. melitensis wild type 16 M was recovered from spleens and livers in greater numbers than B. ...

To determine the effects of straw incorporation on soil nutrients, enzyme activity, and aggregates in tobacco fields, we conducted experiments with different amounts of wheat and maize straw in Zhucheng area of southeast Shandong province for three years (2010–2012). In the final year of experiment (2012), straw incorporation increased soil organic carbon (SOC) and related parameters, and impro...

Catalytic buffering is an advanced method of pH control for the enzyme-based Advanced Catalytic Enzyme System (ACES) and oxidative surety agent decontamination technologies. Ammonia production from urea by urease neutralizes the production of Oalkylphosphonic acids resulting from the hydrolysis of Nerve agents such as Sarin and VX. Fluoride production from Sarin hydrolysis inhibits native ureas...

The Northeastern part of India sprawls over an area of 262 379km2 in the Eastern Himalayan range. This constitutes a biodiversity hotspot with high levels of biodiversity and endemism; unfortunately, is also a poorly known area, especially on its microbial diversity. In this study, we assessed cultivable soil bacterial diversity and distribution from lowlands to highlands (34 to 3 990m.a.s.l.)....

Helicobacter pylori is a human pathogen that infects over 50% of the population worldwide. It is the most important etiologic agent of gastroduodenal ulcers and malignancies. Helicobacter pylori urease enzyme is considered the main factor for the organism's colonization in the gastroduodenal mucosa. Hens immunized with the purified urease produce a highly specific anti-H. pylori urease immunogl...