A partial purification and biochemical characterization of the α-amylase from Streptomyces sp. MSC702 were carried out in this study. The optimum operational conditions for enzyme substrate reaction for amylolytic enzyme activity from the strain were evaluated. The optimum pH, temperature, and incubation period for assaying the enzyme were observed to be 5.0, 55°C, and 30 min, respectively. The...

Atorvastatin calcium, the lipid lowering agent, is taken as a model drug characterized by poor water solubility and bioavailability. In this study an attempt was made for preparation of nanocrystals using high pressure homogenization. A number of stabilizers were included as well as polymers at different concentrations, and the formulations were homogenized for ten cycles at a pressure of 1000 ...

The purpose of this research was to evaluate the effect of penetration enhancers on permeation kinetics of nitrendipine (NTP) through two different skin models. The permeation profile and related kinetics parameters such as activity parameter, diffusion parameter, lag time, relative activity parameter and relative diffusion parameter of NTP was determined in presence of some novel and widely ac...

Two major forms of human carboxylesterase (CES), CES1A and CES2, dominate the pharmacokinetics of most prodrugs such as imidapril and irinotecan (CPT-11). Excipients, largely used as insert vehicles in formulation, have been recently reported to affect drug enzyme activity. The influence of excipients on the activity of CES remains undefined. In this study, the inhibitory effects of 25 excipien...

Objective: To develop Two simple, specific, accurate and precise methods for determination of calcium dobesilate (CD) in the presence of its impurity and / or degradation product namely hydroquinone (HQ). Methods: Method A is an extended ratio subtraction one (EXRSM), while method B is a HPTLC-densitometric one using benzene: methanol: ethyl acetate: sodium lauryl sulphate (7: 2.5: 2: 0.05 v/v/...

I was wondering about the chemical sodium laureth sulfate. I have received several forwarded e-mails about this causing cancer by a lawyer looking into the matter. This chemical was said to be in many shampoos to create a nice lather, but it's actually a garage cleaner and is a cheap substance for the manufacturer, and in the past, only 1 in 2000 would get cancer from this, and now it's about 1...

The present work was conducted on the effect of surfactant on the dissolution rate of ibuprofen tablets. The cationic (Cetyl Trimethyl bromide) and anionic (Sodium dodecyl sulphate) surfactant present in the dissolution media have remarkable effect on the dissolution rate of Ibuprofen. Maximum dissolution was obtained in the presence of anionic surfactant (Sodium dodecyl sulphate) at 0.5% conce...

Different theories have been postulated related to the mechanism of formation and growth of pellets. Some of these theories are derived from experimental results while others are confined to visual observations results obtained from the experiments with some form of tracer technique are regarded as acceptable and convincing. Domperidone pellets are small, free-flowing, spherical or semi-spheric...

Pelletized dosage forms can be prepared by different methods which, in general, are time consuming and labor intensive. The current study was carried out to investigate the feasibility of preparing the spherical pellets of omeprazole by sieving-spheronization. An optimized formulation was also prepared by extrusion-spheronization process to compare the physical parameters between these two meth...

A simple ultra performance liquid chromatographic (UPLC) method has been developed for the simultaneous estimation of Metoprolol (MT), Atorvastatin (AT) and Ramipril (RM) from capsule dosage form. The method was developed using Zorbax XDB-C18 (4.6 mm à 50 mm, 1.8 Îm) column with a mobile phase consisting of 0.06% ortho phosphoric acid in Milli Q water having an ion pair reagent, 0.0045 M Sodi...