ATP citrate lyase was purified by two different procedures from the livers of’ rats first starved and then fed with a fat-deficient and high carbohydrate-glycerol diet. These enzyme preparations were judged homogeneous by sedimentation equilibrium and polyacrylamide gel electrophoresis. The molecular weight of the native enzyme was around 4.4 x 105 as determined hy sedimentation equilibrium. On...

Human blood coagulation Factor XI was purified in 20% yield from plasma by ion exchange chromatography. The specific clotting activity of purified Factor XI was 250 & 20 units/mg implying that its concentration in normal titrated human plasma is 4 pg/ml. The purified Factor XI in its precursor form gave a single protein band on polyacrylamide gels in the presence of sodium dodecyl sulfate with ...

A noncollagenous protein, which is a major component of the extracellular matrix of third trimester fetal epiphyseal cartilage, has been isolated and characterized. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions, the protein exists in the form of its 35,000 molecular weight subunit. Under nonreducing conditions, the protein exists as a 69,000 molecular we...

Cyclooxygenase-2 (COX-2) Renal mesangial cells (RMC) Wild type (WT) Adenovirus (Ad) Tumour necrosis factor α (TNFα) Endothelin-1 (ET-1) Interleukin 1β (IL-1β) Prostaglandin E2 and prostaglandin I2 (PGE2 and PGI2) Annexin V (AV) Fluorescein isothiocyanate (FITC) Propidium iodide (PI) Viable (V), early membrane intact apoptotic (EA) and necrotic (N) Hanks Buffered Saline Solution (HBBS) Phosphate...

Advanced BioMatrix offers a large number of extracellular matrix (ECM) and three dimensional (3D) scaffold products with one of the most extensive Type I collagen product offerings (see Exhibit 1). One analytical method for evaluating the quality, purity and consistency of the products is Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS PAGE electrophoresis). The purpose of the do...

One component of human lymphoblastoid interferon obtained from Namalwa cultures induced by Newcastle disease virus has been purified to a specific activity of 2.5 x 10(8) interferon units per mg of protein (protein content based on amino acid analysis). A single polypeptide species with an apparent molecular weight of 18,500 comigrating with the antiviral activity was observed by sodium dodecyl...

A simple method is described for detecting glycoproteins which had been dot-blotted or electro-blotted on to a polyvinylidene difluoride (PVDF) membrane after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. This method is based on the periodate oxidation of glycoproteins on a PVDF membrane with subsequent staining by the chromophoric hydrazide, 4-N,N-dimethylamino-4'-azobenzene...

Serum samples from patients with various forms of rubella virus infection were tested for antibodies to each of three viral structural proteins by radioimmunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In most sera antibody to E1 protein was the predominant species. Sera from patients with congenital rubella syndrome, however, contained significantly more E2 ant...

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis is a technique that is used to separate proteins in a mixture for the purpose of identifying them individually. Proteins coated in SDS (which gives them a negative charge), are pulled through a thin gel by an electrical current. They separate on the gel based on size--the larger the protein, the slower it moves. Proteins can be stained w...