A defined deletion in the Escherichia coli K-12 sodA gene (encoding manganese-superoxide dismutase) linked to a nontransposable selectable marker was generated by transposon Tn5 insertion in combination with in vitro mutagenesis. This mutant allele was used to replace the wild-type sodA gene in an E. coli clinical isolate of serotype O18ac:K1:H7 by bacteriophage P1 transduction. The O18ac:K1:H7...

Diatomite needs to go through the flux-calcination process in order increase its functionality some specific applications such as filter aids. In this study, effects of thenardite, which was used for first time a flux calcination diatomite, and widely soda ash on industrially important physico-chemical properties diatomite were investigated. Samples taken from two deposits Aegean region Turkey ...

Global warming and depletion of resources have fueled research towards innovative building materials low environmental impact high performance engineering properties. Porous glass-ceramics were synthesized using municipal solid waste incinerator (MSWI) bottom ash (BA) as a starting material. BA was initially milled then vitrified, purification step, by direct microwave heating, characterized gl...

A method for the direct assessment of the dissolved CO2 concentration in supersaturated water is presented that is based on changes to the physical chemistry of soda lime due to uptake of CO2. The approach is an adaptation of a standard method used for the quantifi cation of soil respiration, and was found to agree with pCO2 measurements made using infrared gas analysis. In this method, the sod...

The study aims to compare the lignin extraction methods of OPEFB using different solvents; soda and dioxane solvent, reinforcement effects in polylactic acid (PLA) bio-composite. Different method has been found produce with properties purity. yield also highly dependent on types solvents used process. Soda treatment had shown a good removal 17.4 % yield, but only 8.08 for treatment. In contrast...

BACKGROUND Sarcoidosis is an idiopathic granulomatous disease with pathologic and immunologic features similar to tuberculosis. Routine histologic staining and culture fail to identify infectious agents. An alternative means for investigating a role of infectious agents in human pathogenesis involves molecular analysis of pathologic tissues for microbial nucleic acids, as well as recognition of...

Endospores of Bacillus subtilis are enclosed in a proteinaceous coat which can be differentiated into a thick, striated outer layer and a thinner, lamellar inner layer. We found that the N-terminal sequence of a 25-kDa protein present in a preparation of spore coat proteins matched that of the Mn-dependent superoxide dismutase (SOD) encoded by the sod4 locus. sod4 is transcribed throughout the ...

wood resources, non-wood plants and recycled papers are the common raw materials of papermaking. shortage of raw materials, is a challenging problem in developing pulp and paper industry. in this study, cladophora green algae was used as a new source of cellulosic fiberfor pulping and papermaking.. cladophora algae was cooked with cold soda method and then was mixed with nssc pulp at 10,20 and ...